Wolf C R, Szutowski M M, Ball L M, Philpot R M
Chem Biol Interact. 1978 Apr;21(1):29-43. doi: 10.1016/0009-2797(78)90066-2.
Two forms of rabbit pulmonary cytochrome P-450 have been characterized spectrally and their activities in reconstituted monooxygenase systems investigated. The presence of both microsomal phospholipids and sodium cholate was required to obtain optimum activity. Only one of the cytochromes (I) was active in the N-demethylation of benzphetamine and the O-deethylation of 7-ethoxycoumarin. However, cytochrome II was 20% more active than cytochrome I in the metabolism of benzo[a]pyrene. The profile of the metabolites formed from benzo[a]pyrene indicated that metabolism at the 9 and 10 positions was insignificant in the case of cytochrome I but represented about 40% of the metabolites produced by cytochrome II. The two forms of the cytochrome are present in pulmonary microsomes in approximately equal amounts.
已通过光谱法对兔肺细胞色素P - 450的两种形式进行了表征，并研究了它们在重组单加氧酶系统中的活性。需要微粒体磷脂和胆酸钠同时存在才能获得最佳活性。两种细胞色素中只有一种（I型）在苄非他明的N - 去甲基化和7 - 乙氧基香豆素的O - 去乙基化反应中具有活性。然而，在苯并[a]芘的代谢中，细胞色素II的活性比细胞色素I高20%。由苯并[a]芘形成的代谢产物谱表明，对于细胞色素I而言，在9位和10位的代谢作用不显著，但约占细胞色素II产生的代谢产物的40%。这两种形式的细胞色素在肺微粒体中的含量大致相等。
