Griffin DR, Yang F, Carta G, Gainer JL
Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22903-2442, USA.
Biotechnol Prog. 1998 Jul;14(4):588-93. doi: 10.1021/bp9800586.
Baker's yeast cells entrapped in alginate beads are shown to catalyze reactions in organic solvents when a cofactor regeneration scheme is implemented. This study focused on the reduction of acetophenone to 1-phenylethanol, using baker's yeast as well as a cosubstrate to regenerate the cofactor. The product is a chiral alcohol, and it was desired to maintain a high enantiomeric excess. The effects of parameters such as the addition of a cosubstrate, water content, fermentation time, buffer pH, and bead diameter have been investigated. Such a general process may be quite useful when single enantiomers are needed, as well as for the production of other chemicals.
当实施辅因子再生方案时，包埋在藻酸盐珠中的面包酵母细胞被证明能在有机溶剂中催化反应。本研究重点关注苯乙酮还原为1-苯乙醇的反应，使用面包酵母以及一种共底物来再生辅因子。产物是一种手性醇，希望保持高对映体过量。研究了诸如添加共底物、水含量、发酵时间、缓冲液pH值和珠直径等参数的影响。当需要单一对映体以及生产其他化学品时，这样的一般过程可能非常有用。
