Department of Biological Chemistry, Faculty of Science, Okayama University of Science, 1-1 Ridaicho, 700, Okayama, Japan.
World J Microbiol Biotechnol. 1991 Mar;7(2):219-24. doi: 10.1007/BF00328993.
Immobilized baker's yeast entrapped in calcium alginate beads, ca 1.5 mm diameter, was used more than 10 times in water and reduced ethyl 3-oxobutanoate and ethyl benzoylformate to the corresponding chiral hydroxy esters in good chemical yields and in high enantioselectivities. The biocatalyst was also successfully used in organic/water solvent systems such as hexane/water and acetonitrile/water, and in other systems, particularly in hexane, converted keto esters into their individual chiral hydroxy esters.
固定化啤酒酵母包埋于海藻酸钙珠中，直径约 1.5 毫米，在水相中已重复使用超过 10 次，将乙基 3-氧代丁酸酯和乙基苯甲酰甲酸酯高效、高对映选择性地还原成相应的手性羟基酯。该生物催化剂也成功地应用于有机/水溶剂体系，如正己烷/水和乙腈/水，以及其他体系，特别是在正己烷相中，可将酮酯转化为其各自的手性羟基酯。
