Govitrapong P, Suttitum T, Kotchabhakdi N, Uneklabh T
Neuro-Behavioural Biology Center, Institute of Science and Technology for Research and Development, Mahidol University at Salaya, Nakornpathom 73170, Thailand.
J Pharmacol Exp Ther. 1998 Aug;286(2):883-9.
Conflicting results, both decreased and increased, have been reported concerning the function of T-lymphocytes in heroin addicts. We investigated the alterations of T-lymphocyte proliferative responses and immunophenotypic markers on lymphoid cells in heroin addicts and during different periods of heroin withdrawal in addicted subjects. This study has demonstrated a decrease in the response of T-lymphocytes to 1.2, 2.5, 5 and 10 microg/ml of phytohemagglutinin stimuli in heroin addicts and 1- to 5-day heroin withdrawal subjects compared with controls. Similarly, in an in vitro study, 10(-4), 10(-6) and 10(-8) M concentrations of morphine were shown to suppress 0.6 and 2.5 microg/ml of PHA-stimulated T-lymphocyte obtained from naive subjects. This inhibitory effect of morphine on PHA stimulation was completely abolished by 100 microM naloxone. The immunological parameters of total T-lymphocytes (CD3), T-helper cells (CD4), cytotoxic T-cells (CD8), B-cells and natural killer cells that are the immunophenotypic markers studied by flow cytometric analysis were altered in heroin addicts, 15- to 21-day and 6- to 24-month heroin withdrawal subjects, when compared with controls. These results suggest that heroin addicts and short period (15 to 21 days and 6 to 24 months) of heroin withdrawal have decreases in their immune system functioning and that the heroin withdrawal subjects seem to gradually reverse their immunological parameters to normal levels when withdrawal was sustained >/=2 years. This is the first report examining immune function in heroin withdrawal subjects using the "cold turkey" method. The results are beneficial for further study of the mechanism responsible for the opioid-induced changes in immune function.
关于海洛因成瘾者体内T淋巴细胞的功能，有结果降低和升高这两种相互矛盾的报道。我们研究了海洛因成瘾者以及成瘾者在不同戒毒时期淋巴细胞中T淋巴细胞增殖反应和免疫表型标志物的变化。这项研究表明，与对照组相比，海洛因成瘾者以及戒毒1至5天的受试者体内，T淋巴细胞对1.2、2.5、5和10微克/毫升植物血凝素刺激的反应有所降低。同样，在一项体外研究中，10(-4)、10(-6)和10(-8)摩尔浓度的吗啡可抑制从未接触过药物的受试者中获取的、经0.6和2.5微克/毫升PHA刺激的T淋巴细胞。100微摩尔纳洛酮可完全消除吗啡对PHA刺激的这种抑制作用。通过流式细胞术分析研究的免疫表型标志物，即总T淋巴细胞（CD3）、辅助性T细胞（CD4）、细胞毒性T细胞（CD8）、B细胞和自然杀伤细胞的免疫参数，在海洛因成瘾者、戒毒15至21天以及6至24个月的受试者中与对照组相比发生了改变。这些结果表明，海洛因成瘾者以及短期（15至21天和6至24个月）戒毒者的免疫系统功能下降，而且当戒毒持续时间≥2年时，戒毒者的免疫参数似乎会逐渐恢复到正常水平。这是首篇使用“突然戒断法”研究海洛因戒毒者免疫功能的报告。这些结果有助于进一步研究阿片类药物引起免疫功能变化的机制。
