青少年吸毒者免疫细胞、细胞因子及生长激素的变化

[Changes of the immune cells, cytokines and growth hormone in teenager drug addicts].

作者信息

Kuang Ying-min, Zhu Yue-chun, Kuang Ying, Sun Yuan, Hua Chen, He Wen-yi

机构信息

Department of Transplant, the First Affiliated Hospital, Kunming Medical College, Kunming 650032, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Sep;23(9):821-3.

PMID:17825228

Abstract

AIM

To investigate the effect of heroin on the immune function, growth and development in the teenager heroin addicts by measuring their T-lymphocyte subsets, Th1/Th2 cytokines and serum growth hormone.

METHODS

Tlymphocyte subsets of peripheral blood from the teenager heroin addicts were measured by direct microvolume whole blood immunofluorescent staining technique by flow cytometer (FCM). Thl / Th2 cytokines were measured by BD cytometric bead array and serum growth hormone was assayed using the chemiluminescence method in the 20 teenager heroin addicts and 23 healthy teenagers.

RESULTS

The levels of CD3(+), CD3(+) + CD4(+), CD3(+) + CD4(+)/CD3(+)+ CD8(+), Th1 cytokines(IL-2, TNF-alpha and IFN-gamma) and Th2 cytokines(IL-4 and IL-10) reduced significantly in the teenager heroin addicts compared with the healthy control group (P < 0.01 or P < 0.05). The level of Th1 cytokines(IL-2 + TNF-alpha+IFN-gamma) decreased more than that of Th2 cytokines(IL-4 + IL-5 + IL-10)(P < 0.05). The level of serum growth hormone from the teenager heroin addicts was remarkably higher than that in control group (P<0.01).

CONCLUSION

Heroin can inhibit the immunofunction especially the celluar immunity of the teenager heroin addicts. Besides, it can increase the level of serum growth hormone of the teenager heroin addicts.

摘要

目的

通过检测青少年海洛因成瘾者的T淋巴细胞亚群、Th1/Th2细胞因子和血清生长激素，探讨海洛因对其免疫功能、生长发育的影响。

方法

采用流式细胞仪直接微量全血免疫荧光染色技术检测20例青少年海洛因成瘾者和23例健康青少年外周血T淋巴细胞亚群。采用BD细胞计数珠阵列检测Th1/Th2细胞因子，采用化学发光法检测血清生长激素。

结果

与健康对照组相比，青少年海洛因成瘾者的CD3(+)、CD3(+) + CD4(+)、CD3(+) + CD4(+)/CD3(+)+ CD8(+)水平、Th1细胞因子（IL-2、TNF-α和IFN-γ）和Th2细胞因子（IL-4和IL-10）显著降低（P < 0.01或P < 0.05）。Th1细胞因子（IL-2 + TNF-α+IFN-γ）的降低幅度大于Th2细胞因子（IL-4 + IL-5 + IL-10）（P < 0.05）。青少年海洛因成瘾者的血清生长激素水平显著高于对照组（P<0.01）。