Matsumura M, Mori T
Department of Internal Medicine, Juntendo University School of Medicine.
Nihon Ishinkin Gakkai Zasshi. 1998;39(3):167-71. doi: 10.3314/jjmm.39.167.
We isolated two strains of Aspergillus flavus from a lung lesion and a skin lesion at autopsy from a patient with acute myelogenous leukemia complicated with fungal infection. An attempt was made to detect aflatoxins in culture filtrates of those isolates and the tissue extract of the lung lesion through the techniques of thin-layer chromatography (TLC), densitometry and high-performance liquid chromatography (HPLC). Aflatoxins B1, B2 and M1 were demonstrated in all of these materials qualitatively and quantitatively. The concentrations of aflatoxins in the cultures of the isolates and in the lung lesion extract determined by HPLC were aflatoxin B1: 11.715 microg/ml (lung isolate), 21.383 micro g/ml (skin isolate), 0.635 microg/g (lung extract), aflatoxin B2: 0.341 microg/ml (lung isolate), 0.577 micro g/ml (skin isolate), 0.0273 microg/g (lung extract) and aflatoxin M1: 0.277 microg/ml (lung isolate), 0.491 micro g/ml (skin isolate), 0.0525 microg/g (lung extract), respectively. B1, known as the most toxic among the aflatoxin group, showed the highest concentration through these experiments. This case may be considered as the first to detect aflatoxins in autopsied materials associated with A. flavus infection.
我们从一名患有急性髓性白血病并发真菌感染患者的尸检肺病变和皮肤病变中分离出两株黄曲霉。尝试通过薄层色谱法（TLC）、光密度测定法和高效液相色谱法（HPLC）检测这些分离株的培养滤液和肺病变组织提取物中的黄曲霉毒素。黄曲霉毒素B1、B2和M1在所有这些材料中均得到定性和定量证实。通过HPLC测定的分离株培养物和肺病变提取物中的黄曲霉毒素浓度分别为：黄曲霉毒素B1：11.715微克/毫升（肺分离株）、21.383微克/毫升（皮肤分离株）、0.635微克/克（肺提取物）；黄曲霉毒素B2：0.341微克/毫升（肺分离株）、0.577微克/毫升（皮肤分离株）、0.0273微克/克（肺提取物）；黄曲霉毒素M1：0.277微克/毫升（肺分离株）、0.491微克/毫升（皮肤分离株）、0.0525微克/克（肺提取物）。在黄曲霉毒素组中，已知毒性最强的B1在这些实验中显示出最高浓度。该病例可能被认为是首例在与黄曲霉感染相关的尸检材料中检测到黄曲霉毒素的病例。
