Fulop V, Colitti C V, Genest D, Berkowitz R S, Yiu G K, Ng S W, Szepesi J, Mok S C
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Oncogene. 1998 Jul 30;17(4):419-24. doi: 10.1038/sj.onc.1201955.
Gestational trophoblastic diseases comprise a spectrum of interrelated diseases including partial mole, complete mole and gestational choriocarcinoma. Using reverse transcriptase PCR (RT-PCR) analysis, we identified higher levels of DOC-2/hDab2 expression in the normal trophoblast cells in culture than in choriocarcinoma cell lines. Subsequent study using immunohistochemistry showed high levels of DOC-2/hDab2 protein expression in normal trophoblast tissues but significantly lower levels of expression in gestational trophoblastic disease tissues, particularly in complete mole and choriocarcinoma. When DOC-2/hDab2 was transfected into the choriocarcinoma cell lines, Jar, JEG and BeWo, the stable transfectants showed significantly reduced growth rate in culture. These data suggest that down regulation of DOC-2/hDab2 may play an important role in the development of gestational trophoblastic diseases.
妊娠滋养细胞疾病包括一系列相互关联的疾病，如部分性葡萄胎、完全性葡萄胎和妊娠绒毛膜癌。通过逆转录聚合酶链反应（RT-PCR）分析，我们发现培养的正常滋养层细胞中DOC-2/hDab2的表达水平高于绒毛膜癌细胞系。随后使用免疫组织化学的研究表明，正常滋养层组织中DOC-2/hDab2蛋白表达水平较高，但在妊娠滋养细胞疾病组织中表达水平显著较低，尤其是在完全性葡萄胎和绒毛膜癌中。当将DOC-2/hDab2转染到绒毛膜癌细胞系Jar、JEG和BeWo中时，稳定转染体在培养中的生长速率显著降低。这些数据表明，DOC-2/hDab2的下调可能在妊娠滋养细胞疾病的发生发展中起重要作用。
