Yu Q, Ottmann M, Pechoux C, Le Grice S, Darlix J L
LaboRetro, Unité de Virologie Humaine INSERM U412, Ecole Normale Supérieure de Lyon, 69364 Lyon Cedex 07, France.
J Virol. 1998 Sep;72(9):7676-80. doi: 10.1128/JVI.72.9.7676-7680.1998.
This report describes the effects of mutating highly conserved residues in the primer grip domain of human immunodeficiency virus type 1 reverse transcriptase (RT) on virus formation and infectivity. Among a series of RT mutant viruses, three (M230A, L234D, and W239A) were found to be noninfectious or very poorly infectious. Our data indicate that these mutations in RT caused severe defects in proviral DNA synthesis. Interestingly, assembly and maturation of mutant virus M230A were similar to those of the wild type, while mutants L234D and W239A showed impaired maturation. The immature morphology of RT mutants L234D and W239A is due at least in part to premature cleavage of the gag-pol precursor, prior to virion budding, indicating that intracellular stability of Pr160(gag-pol) is of key importance during virus assembly.
本报告描述了人免疫缺陷病毒1型逆转录酶（RT）引物结合域中高度保守残基的突变对病毒形成和感染性的影响。在一系列RT突变病毒中，发现三种（M230A、L234D和W239A）无感染性或感染性很差。我们的数据表明，RT中的这些突变导致前病毒DNA合成严重缺陷。有趣的是，突变病毒M230A的组装和成熟与野生型相似，而突变体L234D和W239A的成熟受损。RT突变体L234D和W239A的不成熟形态至少部分归因于gag-pol前体在病毒粒子出芽前的过早切割，这表明Pr160（gag-pol）的细胞内稳定性在病毒组装过程中至关重要。
