Hayward S W, Haughney P C, Rosen M A, Greulich K M, Weier H U, Dahiya R, Cunha G R
Department of Anatomy, University of California, San Francisco 94143-0540, USA.
Differentiation. 1998 Jul;63(3):131-40. doi: 10.1046/j.1432-0436.1998.6330131.x.
Tissue recombinants composed of adult human prostatic epithelium (hPrE) and rat urogenital sinus mesenchyme (rUGM) were grafted beneath the renal capsule of athymic rodent hosts. The pseudostratified human epithelium initially became multilayered, solid epithelial cords emerged, grew into the surrounding mesenchyme and canalized to regenerate a pseudostratified epithelium. Basal cells expressed cytokeratins 5 and 14, while luminal cells expressed cytokeratins 8 and 18, prostate specific antigen and prostatic acid phosphatase. The rat mesenchymal component differentiated into thick sheets of smooth muscle, characteristic of the human but not the rat prostate. These findings indicate that epithelial-mesenchymal interactions were reciprocal. Rat UGM induced adult hPrE to form new ductal-acinar tissue, involving epithelial proliferation, ductal branching morphogenesis and functional cytodifferentiation. Concurrently the epithelium dictated smooth muscle differentiation and patterning. Species-specific reverse transcriptase polymerase chain reaction SC (RT-PCR) analysis of the tissue recombinants was performed to separately examine the expression of epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), epidermal growth factor receptor (EGFR), TGF-beta 1, and TGF-beta 3 in the epithelium, stroma and host components of the graft. All of these genes, except TGF-beta 1, were expressed in all three tissues. Human TGF-beta 1 was not detected, indicating that this gene was not expressed in human prostatic epithelium but was present in stroma.
将由成人前列腺上皮（hPrE）和大鼠泌尿生殖窦间充质（rUGM）组成的组织重组体移植到无胸腺啮齿动物宿主的肾被膜下。假复层人上皮最初变为多层，出现实性上皮索，长入周围间充质并形成管道以再生假复层上皮。基底细胞表达细胞角蛋白5和14，而腔面细胞表达细胞角蛋白8和18、前列腺特异性抗原和前列腺酸性磷酸酶。大鼠间充质成分分化为厚层平滑肌，这是人类前列腺而非大鼠前列腺的特征。这些发现表明上皮-间充质相互作用是相互的。大鼠UGM诱导成人hPrE形成新的导管-腺泡组织，涉及上皮增殖、导管分支形态发生和功能性细胞分化。同时，上皮决定平滑肌的分化和模式。对组织重组体进行物种特异性逆转录聚合酶链反应（RT-PCR）分析，以分别检测表皮生长因子（EGF）、转化生长因子-α（TGF-α）、表皮生长因子受体（EGFR）、TGF-β1和TGF-β3在移植物的上皮、基质和宿主成分中的表达。除TGF-β1外，所有这些基因在所有三种组织中均有表达。未检测到人类TGF-β1，表明该基因在人前列腺上皮中不表达，但存在于基质中。
