Hayashi N, Cunha G R, Parker M
Anatomy Department, University of California, San Francisco 94143.
Epithelial Cell Biol. 1993 Apr;2(2):66-78.
Adult rodent (rat and mouse) prostatic ducts (PR) were recombined heterospecifically with fetal urogenital sinus mesenchyme (UGM) (mouse UGM plus rat PR or rat UGM plus mouse PR), and the resultant tissue recombinants were grafted under the renal capsule of male athymic mice. For recombination with mouse UGM the ductal tips of each of the 4 rat prostatic lobes [ventral (VP), lateral type 1 (L1), lateral type 2 (L2), and dorsal prostate (DP)] were used. For recombination with rat UGM the ductal tips of each of the 2 mouse prostatic lobes [ventral (VP) and dorso-lateral prostate (DLP)] were used. After 1 month of growth in vivo, the DNA content of UGM+PR recombinants increased substantially (6.1- to 76.8-fold increase) over the combined DNA content of the isolated UGM and PR prior to grafting. Immunocytochemical, polyacrylamide gel electrophoretic and Western blot analyses demonstrated that irrespective of the initial source of the adult prostatic duct, the epithelium of UGM + PR recombinants continued to express its normal lobe-specific secretory proteins as well as secretory proteins specific to other prostatic lobes. For example, rat ventral and lateral type 2 prostate do not normally express DP-1, a rat dorsal-prostatic-specific protein, but after recombination with mouse UGM the induced prostatic epithelium expressed DP-1 as well as C3, a rat ventral-prostatic-specific protein. Sensitive reverse transcriptase-polymerase chain reaction techniques (RT-PCR) verified the expression of mRNA for C3 and DP-1 in such tissue recombinants. Analogous results were obtained for UGM + PR tissue recombinants constructed with prostatic ductal tips from rat L1, L2 and DP and mouse VP and DLP. These findings demonstrate that adult rodent prostatic epithelium retains a responsiveness to its connective tissue environment, and that fetal UGM can permissively induce prostatic ductal growth and morphogenesis while instructively inducing the expression of a new spectrum of prostatic secretory proteins.
将成年啮齿动物（大鼠和小鼠）的前列腺导管（PR）与胎儿泌尿生殖窦间充质（UGM）进行异种重组（小鼠UGM加大鼠PR或大鼠UGM加小鼠PR），然后将所得的组织重组体移植到雄性无胸腺小鼠的肾包膜下。与小鼠UGM重组时，使用了4个大鼠前列腺叶[腹侧（VP）、外侧1型（L1）、外侧2型（L2）和背侧前列腺（DP）]中每个叶的导管尖端。与大鼠UGM重组时，使用了2个小鼠前列腺叶[腹侧（VP）和背外侧前列腺（DLP）]中每个叶的导管尖端。在体内生长1个月后，UGM + PR重组体的DNA含量比移植前分离的UGM和PR的总DNA含量大幅增加（增加6.1至76.8倍）。免疫细胞化学、聚丙烯酰胺凝胶电泳和蛋白质印迹分析表明，无论成年前列腺导管的初始来源如何，UGM + PR重组体的上皮细胞都继续表达其正常的叶特异性分泌蛋白以及其他前列腺叶特有的分泌蛋白。例如，大鼠腹侧和外侧2型前列腺通常不表达DP-1（一种大鼠背侧前列腺特异性蛋白），但与小鼠UGM重组后，诱导的前列腺上皮细胞既表达DP-1，也表达C3（一种大鼠腹侧前列腺特异性蛋白）。灵敏的逆转录聚合酶链反应技术（RT-PCR）证实了此类组织重组体中C3和DP-1的mRNA表达。用大鼠L1、L2和DP以及小鼠VP和DLP的前列腺导管尖端构建的UGM + PR组织重组体也得到了类似结果。这些发现表明，成年啮齿动物前列腺上皮细胞对其结缔组织环境仍具有反应性，胎儿UGM可以允许性地诱导前列腺导管生长和形态发生，同时指导性地诱导新的一系列前列腺分泌蛋白的表达。
