用于测定红细胞中卤泛群对映体及其主要手性去丁基代谢物的手性色谱法。
Chiral chromatographic method to determine the enantiomers of halofantrine and its main chiral desbutyl metabolite in erythrocytes.
作者信息
Gorichon E, Martin C, Bangchang K N, Karbwang J, Thuillier A, Farinotti R, Gimenez F
机构信息
Hôpital Pitié Salpêtrière, Service Pharmacie-Pharmacocinétique, Paris, France.
出版信息
J Chromatogr B Biomed Sci Appl. 1998 Aug 7;712(1-2):259-62. doi: 10.1016/s0378-4347(98)00151-0.
We describe a direct liquid chromatographic method with spectrofluorimetric detection to quantify the two enantiomers of halofantrine and the two enantiomers of its main chiral N-monodesbutylated metabolite in erythrocyte pellets. The method involves a Chiralpak AD column and a rapid one-step extraction procedure with acetonitrile. The method was validated for the four enantiomers within the range 0-1000 ng/ml. The absence of stereoconversion was studied in samples stored frozen for up to eight months. The optical rotation of the halofantrine and metabolite enantiomers was determined after separation on a semi-preparative Chiralcel OD column with polarimetric detection.
我们描述了一种采用荧光检测的直接液相色谱法，用于定量测定红细胞沉淀中卤泛群的两种对映体及其主要手性N-单去丁基代谢物的两种对映体。该方法采用Chiralpak AD柱，并使用乙腈进行快速一步萃取。该方法在0-1000 ng/ml范围内对四种对映体进行了验证。研究了冷冻保存长达八个月的样品中是否存在立体转化。在使用旋光检测的半制备型Chiralcel OD柱上分离后，测定了卤泛群和代谢物对映体的旋光度。
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