Rigatto S Z, Collares E F
Departamento de Pediatria, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Brasil.
Braz J Med Biol Res. 1998 Apr;31(4):515-8. doi: 10.1590/s0100-879x1998000400006.
The objective of the present study was to evaluate the response of rats suffering from moderate renal insufficiency to bacterial lipopolysaccharide (LPS, or endotoxin). The study involved 48 eight-week-old male SPF Wistar rats (175-220 g) divided into two groups of 24 animals each. One group underwent 5/6 nephrectomy while the other was sham-operated. Two weeks after surgery, the animals were further divided into two subgroups of 12 animals each and were fasted for 20 h but with access to water ad libitum. One nephrectomized and one sham-treated subgroup received E. coli LPS (25 micrograms/kg, i.v.) while the other received a sterile, pyrogen-free saline solution. Gastric retention (GR) was determined 10 min after the orogastric infusion of a standard saline test meal labeled with phenol red (6 mg/dl). The gastric emptying of the saline test meal was studied after 2 h. Renal function was evaluated by measuring the plasma levels of urea and creatinine. The levels of urea and creatinine in 5/6 nephrectomized animals were two-fold higher than those observed in the sham-operated rats. Although renal insufficiency did not change gastric emptying (median %GR = 26.6 for the nephrectomized subgroup and 29.3 for the sham subgroup), LPS significantly retarded the gastric emptying of the sham and nephretomized groups (median %GR = 42.0 and 61.0, respectively), and was significantly greater (p < 0.01) in the nephrectomized rats. We conclude that gastric emptying in animals suffering from moderate renal insufficiency is more sensitive to the action of LPS than in sham animals.
本研究的目的是评估中度肾功能不全大鼠对细菌脂多糖（LPS，即内毒素）的反应。该研究涉及48只8周龄雄性无特定病原体（SPF）的Wistar大鼠（体重175 - 220克），分为两组，每组24只动物。一组接受5/6肾切除术，另一组进行假手术。术后两周，将动物进一步分为两个亚组，每组12只，禁食20小时，但可随意饮水。一个肾切除亚组和一个假手术亚组接受大肠杆菌LPS（25微克/千克，静脉注射），而另一亚组接受无菌、无热原的生理盐水溶液。在经口胃内注入用酚红标记的标准盐水试验餐（6毫克/分升）10分钟后测定胃潴留（GR）。2小时后研究盐水试验餐的胃排空情况。通过测量血浆尿素和肌酐水平评估肾功能。5/6肾切除动物的尿素和肌酐水平比假手术大鼠高两倍。虽然肾功能不全未改变胃排空（肾切除亚组的GR中位数百分比 = 26.6，假手术亚组为29.3），但LPS显著延迟了假手术组和肾切除组的胃排空（GR中位数百分比分别为42.0和61.0），且在肾切除大鼠中显著更高（p < 0.01）。我们得出结论，中度肾功能不全动物的胃排空对LPS作用的敏感性高于假手术动物。
