Blatt S L, Takahashi R N
Departamento de Farmacologia, Universidade Federal de Santa Catarina, Florianópolis, Brasil.
Braz J Med Biol Res. 1998 Apr;31(4):555-9. doi: 10.1590/s0100-879x1998000400013.
Post-training intracerebroventricular administration of procaine (20 micrograms/microliter) and dimethocaine (10 or 20 micrograms/microliter), local anesthetics of the ester class, prolonged the latency (s) in the retention test of male and female 3-month-old Swiss albino mice (25-35 g body weight; N = 140) in the elevated plus-maze (mean +/- SEM for 10 male mice: control = 41.2 +/- 8.1; procaine = 78.5 +/- 10.3; 10 micrograms/microliter dimethocaine = 58.7 +/- 12.3; 20 micrograms/microliter dimethocaine = 109.6 +/- 5.73; for 10 female mice: control = 34.8 +/- 5.8; procaine = 55.3 +/- 13.4; 10 micrograms/microliter dimethocaine = 59.9 +/- 12.3 and 20 micrograms/microliter dimethocaine = 61.3 +/- 11.1). However, lidocaine (10 or 20 micrograms/microliter), an amide class type of local anesthetic, failed to influence this parameter. Local anesthetics at the dose range used did not affect the motor coordination of mice exposed to the rota-rod test. These results suggest that procaine and dimethocaine impair some memory process(es) in the plus-maze test. These findings are interpreted in terms of non-anesthetic mechanisms of action of these drugs on memory impairment and also confirm the validity of the elevated plus-maze for the evaluation of drugs affecting learning and memory in mice.
训练后，向3个月大的雄性和雌性瑞士白化小鼠（体重25 - 35克；N = 140）脑室内注射酯类局部麻醉剂普鲁卡因（20微克/微升）和地美卡因（10或20微克/微升），可延长其在高架十字迷宫中记忆测试的潜伏期（秒）（10只雄性小鼠的平均值±标准误：对照组 = 41.2 ± 8.1；普鲁卡因 = 78.5 ± 10.3；10微克/微升地美卡因 = 58.7 ± 12.3；20微克/微升地美卡因 = 109.6 ± 5.73；10只雌性小鼠：对照组 = 34.8 ± 5.8；普鲁卡因 = 55.3 ± 13.4；10微克/微升地美卡因 = 59.9 ± 12.3；20微克/微升地美卡因 = 61.3 ± 11.1）。然而，酰胺类局部麻醉剂利多卡因（10或20微克/微升）未能影响该参数。所用剂量范围内的局部麻醉剂未影响接受转棒试验小鼠的运动协调性。这些结果表明，普鲁卡因和地美卡因在十字迷宫试验中损害了某些记忆过程。这些发现根据这些药物对记忆损害的非麻醉作用机制进行了解释，也证实了高架十字迷宫在评估影响小鼠学习和记忆的药物方面的有效性。
