Bianchi A, Ferchal F, Lejunter J, Scieux C
Laboratoire de Bactériologie Virologie-Hygiène, Hôpital Saint-Louis, Université Paris VII, France.
New Microbiol. 1998 Jul;21(3):221-32.
The aim of this study was to investigate the effects of infection of monocytic cells with both the human immunodeficiency type 1 virus (HIV-1) and Chlamydia trachomatis on the replication of each pathogen. U-937 cells, chronically infected with HIV-1 (strain LaVLai), either induced to differentiate into immature macrophage-like cells by 32 pM 12-O-tetradecanol phorbol-13-acetate or uninduced, were superinfected with C. trachomatis serovar L2. Both HIV-1 infection and differentiation rendered the U-937 cells highly susceptible to C. trachomatis lytic infection. Differentiation and superinfection of HIV-1-infected cells with C. trachomatis both affected cell viability and reduced viral production in vitro. RT activity was one tenth the original value after differentiation of HIV-1-infected cells, one twentieth the original value after superinfection with C. trachomatis, and one hundredth the original value after differentiation and superinfection with C. trachomatis.
本研究的目的是调查1型人类免疫缺陷病毒（HIV-1）和沙眼衣原体感染单核细胞对每种病原体复制的影响。用32 pM 12-O-十四烷酰佛波醇-13-乙酸盐诱导长期感染HIV-1（LaVLai株）的U-937细胞分化为未成熟的巨噬细胞样细胞，或不进行诱导，然后用沙眼衣原体L2血清型进行超感染。HIV-1感染和分化均使U-937细胞对沙眼衣原体溶细胞感染高度敏感。HIV-1感染细胞的分化和用沙眼衣原体进行超感染均影响细胞活力并降低体外病毒产生。HIV-1感染细胞分化后逆转录酶（RT）活性为原始值的十分之一，用沙眼衣原体超感染后为原始值的二十分之一，分化并用沙眼衣原体超感染后为原始值的百分之一。
