Gallagher D S, Yang Y P, Burzlaff J D, Womack J E, Stelly D M, Davis S K, Taylor J F
Department of Animal Science, Texas A&M University, College Station 77843, USA.
Anim Genet. 1998 Apr;29(2):130-4. doi: 10.1046/j.1365-2052.1998.00239.x.
A bovine bacterial artificial chromosome (BAC) library was screened for the presence of eight type I anchor loci previously used within hybrid somatic cells and an interspecies hybrid backcross to construct a genome map of bovine chromosome 19 (BTA19). Six out of eight loci were identified in the BAC library (NF1, CRYB1, CHRNB1, TP53, GH1 and P4HB). The BACs were then used in single-colour fluorescence in situ hybridization (FISH) to assign these genes to BTA19 band locations. Gene order was determined by single-colour FISH, and was confirmed by dual-colour FISH to mitotic and meiotic chromosomes. The order, centromere-NF1-CRYB1-CHRNB1-TP53-GH1-P4HB, was in agreement with the order determined by linkage analyses. In addition, the order of CHRNB1 and TP53, previously unresolved by linkage analyses, was established. These data provide high-resolution cytogenetic anchorage of the BTA19 genome map from chromosome bands 14-22.
对一个牛细菌人工染色体（BAC）文库进行筛选，以寻找先前在杂种体细胞和种间杂交回交中用于构建牛19号染色体（BTA19）基因组图谱的8个I型锚定基因座。在BAC文库中鉴定出了8个基因座中的6个（NF1、CRYB1、CHRNB1、TP53、GH1和P4HB）。然后将这些BAC用于单色荧光原位杂交（FISH），以将这些基因定位到BTA19的带位置。通过单色FISH确定基因顺序，并通过对有丝分裂和减数分裂染色体的双色FISH进行确认。顺序为着丝粒 - NF1 - CRYB1 - CHRNB1 - TP53 - GH1 - P4HB，与连锁分析确定的顺序一致。此外，还确定了先前连锁分析未解决的CHRNB1和TP53的顺序。这些数据为BTA19基因组图谱从14 - 22号染色体带提供了高分辨率的细胞遗传学定位。
