Gallagher D S, Schläpfer J, Burzlaff J D, Womack J E, Stelly D M, Davis S K, Taylor J F
Department of Animal Science, Texas A&M University, College Station 77843, USA.
Chromosome Res. 1999;7(2):115-9. doi: 10.1023/a:1009243015544.
A bovine bacterial artificial chromosome (BAC) library was screened for the presence of six genes (IL2RA, VIM, THBD, PLC-II, CSNK2A1 and TOP1) previously assigned to human chromosomes 10 or 20 (HSA10 or HSA20). Four of the genes were found represented in the bovine BAC library by at least one clone. The identified BAC clones were used as probes in single-color fluorescence in-situ hybridization (FISH) to determine the chromosomal band location of each gene. As predicted by the human/bovine comparative map and comparative chromosome painting analysis, the four genes mapped to bovine chromosome 13 (BTA13). Dual-color FISH was then used to integrate these four type I markers into the existing BTA13 genome map. These FISH results anchor the BTA13 genome map from bands 14-23, and confirm the presence of a conserved HSA10 homologous synteny group on BTA13 centromeric to a HSA20 homologous segment.
对一个牛细菌人工染色体（BAC）文库进行筛选，以查找先前定位到人类10号或20号染色体（HSA10或HSA20）上的六个基因（IL2RA、VIM、THBD、PLC-II、CSNK2A1和TOP1）。发现其中四个基因在牛BAC文库中至少有一个克隆代表。将鉴定出的BAC克隆用作单颜色荧光原位杂交（FISH）的探针，以确定每个基因的染色体带位置。正如人类/牛比较图谱和比较染色体涂染分析所预测的那样，这四个基因定位到牛13号染色体（BTA13）。然后使用双色FISH将这四个I型标记整合到现有的BTA13基因组图谱中。这些FISH结果确定了BTA13基因组图谱从14至23带的位置，并证实了在BTA13上存在一个与HSA20同源片段着丝粒相连的保守HSA10同源同线群。
