用尖孢镰刀菌的nia基因对灰玫瑰青霉硝酸还原酶突变体进行转化。

Transformation of Penicillium griseoroseum nitrate reductase mutant with the nia gene from Fusarium oxysporum.

作者信息

de Queiroz M V, Barros A O, de Barros E G, Guimarães V, de Araújo E F

机构信息

Departamento de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, MG, Brazil.

出版信息

Can J Microbiol. 1998 May;44(5):487-9. doi: 10.1139/w98-022.

DOI:10.1139/w98-022

PMID:9699303

Abstract

A heterologous transformation system for Penicillium griseoroseum has been developed. This system is based on nia, the structural gene from Fusarium oxysporum encoding nitrate reductase. Penicillium griseoroseum niaD mutants have been selected from chlorate-resistant colonies. Among 24 chlorate-resistant colonies analyzed, 2 were confirmed to be niaD mutants. Transformation frequencies of 8 transformants/microgram of DNA were obtained. DNA hybridization analyses of five transformants showed distinct integration patterns of the plasmid and in all of them the integration occurred at tandem arrays. The transformation system established in this work will be useful for genetic studies of the pectinolytic complex genes from P. griseoroseum.

摘要

已开发出一种用于玫瑰色青霉的异源转化系统。该系统基于nia，它是尖孢镰刀菌编码硝酸还原酶的结构基因。已从耐氯酸盐菌落中筛选出玫瑰色青霉niaD突变体。在分析的24个耐氯酸盐菌落中，有2个被确认为niaD突变体。获得了每微克DNA 8个转化体的转化频率。对5个转化体的DNA杂交分析显示了质粒的不同整合模式，并且在所有转化体中，整合均以串联阵列形式发生。这项工作中建立的转化系统将有助于对玫瑰色青霉果胶分解复合基因进行遗传学研究。