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Integrative and replicative transformation of Penicillium canescens with a heterologous nitrate-reductase gene.

作者信息

Aleksenko A Y, Makarova N A, Nikolaev I V, Clutterbuck A J

机构信息

Genetics Laboratories, University of Glasgow, Scotland, UK.

出版信息

Curr Genet. 1995 Oct;28(5):474-7. doi: 10.1007/BF00310818.

DOI:10.1007/BF00310818
PMID:8575022
Abstract

A wild isolate of Penicillium canescens was subjected to mutagenesis, and 150 chlorate-resistant mutants were isolated and classified in respect of their ability to utilize various nitrogen sources. Strains supposedly deficient in nitrate reductase have been transformed with the nitrate-reductase gene from Aspergillus niger. Transformation probably occurred by non-homologous integration of the transforming vector into the chromosome. Co-transformation with the AMA 1 replicating element from A. nidulans enhanced transformation frequency up to 2000-fold, and was shown to result in autonomous maintenance of replicating concatenates, one of which was re-isolated by transformation of E. coli.

摘要

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Curr Genet. 1993 Dec;24(6):520-4. doi: 10.1007/BF00351716.
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Mol Gen Genet. 1994 Feb;242(4):467-71. doi: 10.1007/BF00281798.
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Cointegration of transforming DNAs in Aspergillus nidulans: a model using autonomously-replicating plasmids.
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6
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