Abou Hashieh I, Franquin J C, Cosset A, Dejou J, Camps J
IMEB EA 1787, Faculté d'Odontologie, Marseille, France.
J Dent. 1998 Jul-Aug;26(5-6):473-7. doi: 10.1016/s0300-5712(98)00019-0.
Dentine modifies pulpward diffusion of monomers leaching from restorative materials. Thus, remaining dentine thickness must be taken into account during in vitro cytotoxicity tests. This in vitro study was designed to determine the influence of dentine permeability on the outcome of a cytotoxicity test.
Dentine slices were made from 36 human third permanent molar teeth. The 36 dentine slices were divided into two groups according to their hydraulic conductance: high or low hydraulic conductance. The cytotoxicity of four dentine bonding agents of similar cytotoxicity was tested on dentine slices from each group. Four dilutions of the experimental culture medium were tested: undiluted, 1:2, 1:10 and 1:100. An analysis of variance was used to compare the cytotoxicity of the dentine bonding agents tested on high versus low hydraulic conductance.
The cytotoxicity of the high hydraulic conductance (Lp) group was higher than that of low Lp group when tested with the undiluted test culture medium (p = 0.001). No difference was obtained with the 1:2, 1:10, 1:100 dilutions.
Under the conditions of the study, the dentine bonding resins were more cytotoxic when applied onto dentine slices of high hydraulic conductance.
牙本质会改变从修复材料中渗出的单体向牙髓方向的扩散。因此，在体外细胞毒性试验中必须考虑剩余牙本质厚度。本体外研究旨在确定牙本质渗透性对细胞毒性试验结果的影响。
从36颗人类第三恒磨牙制备牙本质切片。根据其水力传导率将36片牙本质切片分为两组：高水力传导率组或低水力传导率组。在每组的牙本质切片上测试四种细胞毒性相似的牙本质粘结剂的细胞毒性。测试了实验培养基的四种稀释度：未稀释、1:2、1:10和1:100。采用方差分析比较在高水力传导率和低水力传导率下测试的牙本质粘结剂的细胞毒性。
用未稀释的测试培养基测试时，高水力传导率（Lp）组的细胞毒性高于低Lp组（p = 0.001）。在1:2、1:10、1:100稀释度下未发现差异。
在本研究条件下，将牙本质粘结树脂应用于高水力传导率的牙本质切片时，其细胞毒性更大。
