Piao Z, Park C, Park J H, Kim H
Department of Pathology, Yonsei University, College of Medicine, Seoul, Korea.
Int J Cancer. 1998 Aug 21;79(4):356-60. doi: 10.1002/(sici)1097-0215(19980821)79:4<356::aid-ijc8>3.0.co;2-u.
Hepatocellular carcinoma (HCC) frequently shows a loss of heterozygosity (LOH) on chromosome 4q. In order to define the commonly affected region on chromosome 4q for further positional cloning of the putative tumor suppressor gene, we carried out allelic imbalance (AI) studies in 41 HCCs using a panel of 43 microsatellite markers. Thirty-four cases (82.9%) showed AI at one or more loci. Detailed deletion mapping identified 7 independent, frequently deleted regions on this chromosome arm. These were the (1) D4S1615 locus, (2) D4S1598 locus, (3) D4S620 locus, (4) D4S1566 and D4S2979 loci, (5) D4S1617 and D4S1545 loci, (6)D4S1537 locus; and (7) from the D4S2920 to D4S2954 locus. Among these 7 frequently deleted regions, 5 were associated with tumor differentiation. Our results suggest that several putative tumor suppressor genes may be present on chromosome 4q and that the AI of chromosome 4q may play a role in the aggressive progression of HCC.
肝细胞癌（HCC）常显示4号染色体q臂杂合性缺失（LOH）。为了确定4号染色体q臂上常见的受影响区域，以便进一步对假定的肿瘤抑制基因进行定位克隆，我们使用一组43个微卫星标记对41例HCC进行了等位基因不平衡（AI）研究。34例（82.9%）在一个或多个位点显示AI。详细的缺失定位确定了该染色体臂上7个独立的、经常缺失的区域。这些区域分别是：（1）D4S1615位点；（2）D4S1598位点；（3）D4S620位点；（4）D4S1566和D4S2979位点；（5）D4S1617和D4S1545位点；（6）D4S1537位点；以及（7）从D4S2920到D4S2954位点。在这7个经常缺失的区域中，有5个与肿瘤分化相关。我们的结果表明，4号染色体q臂上可能存在几个假定的肿瘤抑制基因，并且4号染色体q臂的AI可能在HCC的侵袭性进展中起作用。
