聚乙二醇-柠檬酸钠双水相体系中青霉素酰化酶分配系数随相比的变化
Variation of penicillin acylase partition coefficient with phase volume ratio in poly(ethylene glycol)-sodium citrate aqueous two-phase systems.
作者信息
Marcos J C, Fonseca L P, Ramalho M T, Cabral J M
机构信息
Instituto de Biotecnologia e Química Fina (Polo de Braga), Universidade do Minho, Campus de Gualtar, Portugal.
出版信息
J Chromatogr B Biomed Sci Appl. 1998 Jun 26;711(1-2):295-9. doi: 10.1016/s0378-4347(97)00633-6.
The influence of phase volume ratio on partition and purification of penicillin acylase from Escherichia coli on poly(ethylene glycol)-sodium citrate aqueous two-phase systems was studied. In PEG 1000 systems both partition coefficients of the enzyme and total protein increased with decreasing phase volume ratio. However, in PEG 3350 containing NaCl, penicillin acylase follows a reverse trend, while total protein behaves in the same way. Implications for protein purification designs are discussed.
研究了相体积比对聚乙二醇-柠檬酸钠双水相体系中大肠杆菌青霉素酰化酶分配和纯化的影响。在聚乙二醇1000体系中，酶和总蛋白的分配系数均随相体积比的降低而增加。然而，在含有氯化钠的聚乙二醇3350体系中，青霉素酰化酶呈现相反的趋势，而总蛋白的行为方式相同。讨论了对蛋白质纯化设计的影响。
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