School of Industrial Technology, Universiti Sains Malaysia, Gelugor, Pulau Pinang 11800, Malaysia.
Halal Products Research Institute, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Molecules. 2020 Nov 16;25(22):5332. doi: 10.3390/molecules25225332.
is a LAB strain which is capable of producing bacteriocin substances to inhibit The aim of this study was to purify a bacteriocin-like inhibitory substance (BLIS) produced by FTDC 1211 using an aqueous impregnated resins system consisting of polyethylene-glycol (PEG) impregnated on Amberlite XAD4. Important parameters influencing on purification of BLIS, such as the molecular weight and concentration of PEG, the concentration and pH of sodium citrate and the concentration of sodium chloride, were optimized using a response surface methodology. Under optimum conditions of 11% (/) of PEG 4000 impregnated Amberlite XAD4 resins and 2% (/) of sodium citrate at pH 6, the maximum purification factor (3.26) and recovery yield (82.69% ± 0.06) were obtained. These results demonstrate that AIRS could be used as an alternate purification system in the primary recovery step.
是一种 LAB 菌株，能够产生细菌素物质来抑制。本研究的目的是使用包含聚乙二醇（PEG）浸渍在 Amberlite XAD4 上的水浸渍树脂系统来纯化由 产生的细菌素样抑制物质（BLIS）。使用响应面法优化了影响 BLIS 纯化的重要参数，如 PEG 的分子量和浓度、柠檬酸钠的浓度和 pH 值以及氯化钠的浓度。在最佳条件下，使用 11%（/）PEG 4000 浸渍的 Amberlite XAD4 树脂和 2%（/）柠檬酸钠在 pH 6 下，获得了最大的纯化因子（3.26）和回收率（82.69%±0.06）。这些结果表明，AIRS 可以用作初步回收步骤中的替代纯化系统。
