Hyder S M, Chiappetta C, Stancel G M
Department of Integrative Biology, Pharmacology and Physiology, University of Texas Medical School, Houston 77225, USA.
Mol Biol Rep. 1998 Jul;25(3):189-91. doi: 10.1023/a:1006841230054.
We previously identified an estrogen response element in the 3'-flanking region of the c-fos protooncogene [1, 2]. This element, GGTCAnnnCAGCC, has one half-site identical to that of the consensus ERE (GGTCAnnnTGACC) but only limited homology to the second half-site. Because of this non-canonical sequence and atypical location in the 3'-untranslated region of an estrogen target gene, we decided to analyze sequences adjacent to this element for the possible presence of other regulatory elements. We now report that the 635 base pairs downstream of the c-fos ERE contain: (i) an unusual cluster of 7 GGTCA hormone response-like elements; (ii) potential binding sites for other known DNA binding proteins; and (iii) a sequence specific binding site for a non-estrogen receptor protein present in hormone target tissues.
我们先前在原癌基因c-fos的3'侧翼区域鉴定出一个雌激素反应元件[1,2]。该元件GGTCAnnnCAGCC，有一个半位点与共有雌激素反应元件（GGTCAnnnTGACC）的半位点相同，但与第二个半位点只有有限的同源性。由于这种非典型序列以及其在雌激素靶基因3'非翻译区的非典型位置，我们决定分析该元件附近的序列，以寻找可能存在的其他调控元件。我们现在报告，c-fos雌激素反应元件下游的635个碱基对包含：（i）7个GGTCA激素反应样元件的异常簇；（ii）其他已知DNA结合蛋白的潜在结合位点；以及（iii）激素靶组织中存在的一种非雌激素受体蛋白的序列特异性结合位点。
