连续去除组蛋白后染色质颗粒大小和形状的变化
Changes in size and shape of chromatin particles after successive removal of histones.
作者信息
Kaliński A, Toczko K
出版信息
Acta Biochim Pol. 1976;23(2-3):103-8.
The preparations of whole chromatin, chromatin selectively depleted of histone f1, depleted of all lysine-rich histones (f1, f2b, f2a2), and DNA was studied by viscosimetric and light scattering methods. The obtained results were used for calculation of the dimensions and packing ratios of DNA for the preparations studied. The packing ratio in whole chromatin is 7.2 and is almost unaffected by selective removal of histone f1 (6.9), but decreases on successive removal of the remaining four histones, the decrease being dependent more on the quantity than the kind of the dissociated histones.
采用粘度测定法和光散射法研究了全染色质、选择性去除组蛋白F1的染色质、去除所有富含赖氨酸组蛋白（F1、F2b、F2a2）的染色质以及DNA的制备物。所得结果用于计算所研究制备物中DNA的尺寸和堆积比。全染色质中的堆积比为7.2，选择性去除组蛋白F1（6.9）对其几乎没有影响，但随着其余四种组蛋白的相继去除而降低，这种降低更多地取决于解离组蛋白的数量而非种类。
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