人胎盘tRNA的制备及其性质
Preparation and properties of tRNA from human placenta.
作者信息
Woźniak M, Dobryszycka W
出版信息
Acta Biochim Pol. 1976;23(2-3):171-6.
PMID:970031
Abstract
- The tRNA preparation obtained from human placenta by phenol extraction and DEAE-cellulose chromatography was homogeneous on ultracentrifugation and showed high amino acid acceptor activity. 2. The analysis of the isoaccepting tRNA by reversed-phase chromatography (RPC-5 system) showed the presence of 5 fractions for glycine and leucine, 3 for tyrosine, alanine and valine, 2 for arginine and 1 for phenylalanine.
摘要
- 通过苯酚抽提和二乙氨基乙基纤维素色谱法从人胎盘中获得的tRNA制剂，在超速离心时是均一的，并且显示出高氨基酸接受活性。2. 通过反相色谱法（RPC - 5系统）对同功tRNA的分析表明，甘氨酸和亮氨酸有5个组分，酪氨酸、丙氨酸和缬氨酸有3个组分，精氨酸有2个组分，苯丙氨酸有1个组分。
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