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关于人转运核糖核酸的研究。I. 胎盘和肝脏转运核糖核酸的快速大规模分离及部分分级分离

Studies on human tRNA. I. The rapid, large scale isolation and partial fractionation of placenta and liver tRNA.

作者信息

Roe B A

出版信息

Nucleic Acids Res. 1975 Jan;2(1):21-42. doi: 10.1093/nar/2.1.21.

DOI:10.1093/nar/2.1.21
PMID:236551
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342809/
Abstract

A procedure for the large scale isolation of mammalian tRNA has been applied to the isolation of several grams of human liver, human placenta, rabbit liver and rat liver tRNA. This procedure entails an initial grinding of the tissue in phenol-sodium acetate at acidic pH, followed by DEAE cellulose chromatography. Procedures are also described for analysis of the purified tRNA on the basis of size, using controlled pore glass bean columns. In addition, the acceptor activity of isolated tRNAs has been determined using both the heterologous and homologous synthetases. The chromatographic profile of individual isoaccepting species using BD cellulose chromatography is shown and the 3' terminal nucleoside content was also determined. The methods described now make it feasible for large scale studies of mammalian tRNA enabling us to better understand the relationships between the structure of mammalian tRNA and its many diversified functions.

摘要

一种大规模分离哺乳动物tRNA的方法已被应用于从几克人类肝脏、人类胎盘、兔肝脏和大鼠肝脏中分离tRNA。该方法首先在酸性pH条件下于苯酚 - 醋酸钠中研磨组织，随后进行DEAE纤维素色谱分离。还介绍了使用可控孔径玻璃珠柱基于大小分析纯化tRNA的方法。此外，已使用异源和同源合成酶测定了分离的tRNA的受体活性。展示了使用BD纤维素色谱法对各个同功受体种类的色谱图谱，并且还测定了3'末端核苷含量。现在所描述的方法使得对哺乳动物tRNA进行大规模研究成为可能，从而使我们能够更好地理解哺乳动物tRNA的结构与其多种多样化功能之间的关系。

相似文献

1
Studies on human tRNA. I. The rapid, large scale isolation and partial fractionation of placenta and liver tRNA.关于人转运核糖核酸的研究。I. 胎盘和肝脏转运核糖核酸的快速大规模分离及部分分级分离
Nucleic Acids Res. 1975 Jan;2(1):21-42. doi: 10.1093/nar/2.1.21.
2
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3
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4
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Protein synthesis in a cell-free system prepared from human placenta. II. pH 5 enzyme inefficiency due to defects in tRNA charging with resulting loss of elongation factor 1.从人胎盘制备的无细胞系统中的蛋白质合成。II. pH 5酶效率低下是由于tRNA充电缺陷导致延伸因子1丢失。
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Change of isoaccepting threonyl-tRNA and contitutively increased level of threonyl-tRNA-synthetase in E. coli.大肠杆菌中同工受体苏氨酰 - tRNA的变化及苏氨酰 - tRNA合成酶的持续增加水平
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Chromatography of Drosophila tRNA on BD-cellulose.果蝇转运核糖核酸在BD-纤维素上的色谱分析。
Can J Biochem. 1973 Jun;51(6):896-902. doi: 10.1139/o73-111.

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本文引用的文献

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A simplified procedure for the preparation of "soluble" RNA from rat liver.一种从大鼠肝脏制备“可溶性”RNA的简化方法。
Biochem Biophys Res Commun. 1962 Jun 19;8:1-3. doi: 10.1016/0006-291x(62)90223-1.
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The preparation of rat-liver soluble ribonucleic acid.大鼠肝脏可溶性核糖核酸的制备
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A fluorescence assay for phenylalanine transfer RNA.苯丙氨酸转运RNA的荧光测定法。
Biochim Biophys Acta. 1968 Oct 29;166(3):714-7. doi: 10.1016/0005-2787(68)90382-1.
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A modified procedure for large-scale preparation of beef liver tRNA.一种用于大规模制备牛肝转移核糖核酸（tRNA）的改良方法。
Anal Biochem. 1970 Sep;37(1):11-20. doi: 10.1016/0003-2697(70)90252-6.
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A procedure for isolating transfer ribonucleic acid from human placenta.一种从人胎盘中分离转移核糖核酸的方法。
Biochim Biophys Acta. 1970 Feb 18;199(2):528-31. doi: 10.1016/0005-2787(70)90097-3.
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Isolation of mammalian transfer RNA.哺乳动物转运核糖核酸的分离
Biochim Biophys Acta. 1969 Nov 19;195(1):13-5. doi: 10.1016/0005-2787(69)90597-8.
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Large scale preparation of rabbit liver tRNA. Isolation of tRNAs specific for methionine, phenylalanine, serine, tryptophan and valine.兔肝tRNA的大规模制备。甲硫氨酸、苯丙氨酸、丝氨酸、色氨酸和缬氨酸特异性tRNA的分离。
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Large-scale preparation of rabbit liver tRNA.兔肝转移核糖核酸的大规模制备
Anal Biochem. 1972 May;47(1):102-8. doi: 10.1016/0003-2697(72)90283-7.
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Human gene expression. I. An aminoacyl-RNA binding system from human placenta.人类基因表达。I. 来自人胎盘的氨酰基RNA结合系统。
Biochem Biophys Res Commun. 1967 Jun 23;27(6):638-43. doi: 10.1016/s0006-291x(67)80082-2.
10
A simple method for large-scale preparation of chick embryo tRNA.一种大规模制备鸡胚转移核糖核酸的简单方法。
Anal Biochem. 1974 Mar;58(1):190-4. doi: 10.1016/0003-2697(74)90457-6.