Dunon D, Allioli N, Vainio O, Ody C, Imhof B A
UMR-CNRS 7622, Université Pierre et Marie Curie, Paris, France.
Dev Comp Immunol. 1998 May-Jun;22(3):279-87. doi: 10.1016/s0145-305x(98)00010-x.
The avian thymus is colonized by three waves of hemopoietic progenitors during embryogenesis. An in vivo thymus reconstitution assay based on intrathymic injection of irradiated chicks showed that cells of para-aortic foci were able to differentiate into T lymphocytes, confirming their putative role in the first wave of thymus colonization. This assay was also used to detect and to characterize T cell progenitors from the bone marrow which are involved in the second and third wave of thymus colonization. In the bone marrow, progenitors that differentiated into T cells were found in a subpopulation that expressed the molecules HEMCAM, c-kit and c128. Engraftment of thymus lobes into thymectomized young chick recipients showed that T cell progenitors are replaced in the thymus by subsequent waves of progenitors after hatching. Finally, analysis of thymocyte differentiation suggested that gamma delta and alpha beta T cells migrate from the thymus to the periphery in alternating waves.
在胚胎发育过程中，鸟类胸腺由三波造血祖细胞定植。基于向受辐照雏鸡胸腺内注射的体内胸腺重建试验表明，主动脉旁灶的细胞能够分化为T淋巴细胞，证实了它们在胸腺定植第一波中的假定作用。该试验还用于检测和鉴定参与胸腺定植第二波和第三波的来自骨髓的T细胞祖细胞。在骨髓中，在表达分子HEMCAM、c-kit和c128的亚群中发现了分化为T细胞的祖细胞。将胸腺叶移植到胸腺切除的年轻雏鸡受体中表明，孵化后，T细胞祖细胞在胸腺中被随后的祖细胞波所取代。最后，胸腺细胞分化分析表明，γδ和αβ T细胞以交替波的形式从胸腺迁移到外周。
