垂体腺苷酸环化酶激活肽-27对离体兔壁细胞中14C-氨基比林蓄积的影响。

Effect of PACAP-27 on 14C-aminopyrine accumulation in isolated rabbit parietal cells.

作者信息

Healey Z V, Bliss P, Edwards J, Arebi N, Beales I L, Calam J

机构信息

Department of Medicine, Imperial College School of Medicine, Hammersmith Hospital, London, United Kingdom.

出版信息

Peptides. 1998;19(6):1111-4. doi: 10.1016/s0196-9781(98)00051-5.

DOI:10.1016/s0196-9781(98)00051-5

PMID:9700763

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is present in gastric mucosa, but its direct effect on parietal cells is unknown. We examined this using 14C-aminopyrine uptake in elutriated rabbit cells. PACAP-27 had no effect on basal cells but significantly increased the response to histamine (10(-4) M) at 10(-9) M and to carbachol (10(-4) M) in the presence of ranitidine (10(-4) M) at 10(-7) M and 10(-8) M. PACAP (6-38), an antagonist of PACAP, inhibited the effect of PACAP-27 on carbachol-stimulated cells. Vasoactive intestinal peptide had no significant effect. In conclusion, PACAP-27 has a direct additive effect on stimulated rabbit parietal cells in vitro.

摘要

垂体腺苷酸环化酶激活多肽（PACAP）存在于胃黏膜中，但其对壁细胞的直接作用尚不清楚。我们使用淘洗的兔细胞中的14C-氨基比林摄取来对此进行研究。PACAP-27对基础细胞无作用，但在10(-9) M时可显著增强对组胺（10(-4) M）的反应，在10(-7) M和10(-8) M时，在雷尼替丁（10(-4) M）存在的情况下可增强对卡巴胆碱（10(-4) M）的反应。PACAP拮抗剂PACAP（6-38）可抑制PACAP-27对卡巴胆碱刺激细胞的作用。血管活性肠肽无显著作用。总之，PACAP-27在体外对受刺激的兔壁细胞有直接的相加作用。