Umeda Y, Okada T
Biochem Biophys Res Commun. 1987 Jul 31;146(2):430-6. doi: 10.1016/0006-291x(87)90547-x.
The effect of CGRP on [14C]-aminopyrine accumulation in isolated parietal cell preparations from guinea-pig fundic mucosa was studied. Parietal cells consisted of 60% of the preparations. [14C]-Aminopyrine accumulation was used as an index of physiological response of parietal cells to secretagogues. CGRP dose-dependently (10(-12)-10(-9) M) inhibited parietal cell aminopyrine accumulation stimulated by histamine (10(-4) M), carbachol (10(-4) M), and pentagastrin (5 X 10(-6) M). The concentration of CGRP exerting half-maximal inhibition of [14C]-aminopyrine accumulation was 8.7 X 10(-11) M for histamine, 9.1 X 10(-11) M for carbachol, and 4.7 X 10(-11) M for pentagastrin. The inhibitory effect was much more potent than cimetidine, pirenzepine or benzotript. CGRP but not cimetidine inhibited DBcAMP stimulated aminopyrine accumulation (IC50 = 7.5 X 10(-11) M). These results suggest that CGRP may exert its inhibitory action on gastric acid secretion by a direct action on the parietal cell or the somatostatin-producing D cell.
研究了降钙素基因相关肽（CGRP）对豚鼠胃底黏膜分离壁细胞制剂中[¹⁴C] - 氨基比林蓄积的影响。壁细胞占制剂的60%。[¹⁴C] - 氨基比林蓄积用作壁细胞对促分泌剂生理反应的指标。CGRP以剂量依赖性方式（10⁻¹² - 10⁻⁹ M）抑制组胺（10⁻⁴ M）、卡巴胆碱（10⁻⁴ M）和五肽胃泌素（5×10⁻⁶ M）刺激的壁细胞氨基比林蓄积。对[¹⁴C] - 氨基比林蓄积产生半数最大抑制作用时，CGRP的浓度对组胺为8.7×10⁻¹¹ M，对卡巴胆碱为9.1×10⁻¹¹ M，对五肽胃泌素为4.7×10⁻¹¹ M。其抑制作用比西咪替丁、哌仑西平或苯佐曲普更有效。CGRP而非西咪替丁抑制二丁酰环磷腺苷（DBcAMP）刺激的氨基比林蓄积（IC₅₀ = 7.5×10⁻¹¹ M）。这些结果表明，CGRP可能通过直接作用于壁细胞或产生生长抑素的D细胞对胃酸分泌发挥抑制作用。
