Suppr超能文献

在大鼠癫痫点燃模型的海马体中,突触素I的mRNA水平升高,但突触素II的mRNA水平未升高。

Increases in mRNA levels for synapsin I but not synapsin II in the hippocampus of the rat kindling model of epilepsy.

作者信息

Morimoto K, Sato K, Sato S, Suemaru S, Sato T, Yamada N, Hayabara T

机构信息

Department of Neuropsychiatry, Faculty of Medicine, Kagawa Medical University, Japan.

出版信息

Seizure. 1998 Jun;7(3):229-35. doi: 10.1016/s1059-1311(98)80041-1.

Abstract

We studied brain synapsin I and II mRNA levels using the amygdala kindling model of epilepsy. There were significant increases in the synapsin I mRNA level in the granule cell layer of the hippocampal bilateral dentate gyrus. One to 8 h after seizures, the level in the dentate gyrus ipsilateral to stimulation increased by 44.2-73.2%, compared with the control level. Of the time points investigated, the greatest increase in expression was observed 8 h after the kindled seizures. Furthermore, the synapsin I mRNA levels in the dentate gyrus contralateral to stimulation increased by 28.0% and 51.1%, 2 and 8 h, respectively, after the kindled seizures. Expression of this mRNA, however, did not change significantly in other areas examined, including CA1, CA2, CA3 and the polymorphic layer of the hippocampus and the perirhinal and temporal cortices. Synapsin II mRNA levels did not change significantly in any of the regions studied for up to 24 h after the seizures and synapsin II was presumed to have little involvement in kindling. We considered the locally elevated synapsin I mRNA levels in the bilateral dentate gyrus associated with kindling indicate that excitatory changes occur in the synaptic circuit in which the dentate granule cells participate. Synapsin I may be involved in the presynaptic molecular mechanisms underlying the neuronal plasticity in kindling.

摘要

我们使用癫痫的杏仁核点燃模型研究了脑突触素I和II的mRNA水平。海马双侧齿状回颗粒细胞层中突触素I的mRNA水平显著升高。癫痫发作后1至8小时,与对照水平相比,刺激同侧齿状回中的水平升高了44.2%至73.2%。在所研究的时间点中,点燃性癫痫发作后8小时观察到表达增加最大。此外,点燃性癫痫发作后2小时和8小时,刺激对侧齿状回中突触素I的mRNA水平分别升高了28.0%和51.1%。然而,该mRNA在其他检查区域,包括海马的CA1、CA2、CA3和多形层以及嗅周皮质和颞叶皮质中,表达没有显著变化。癫痫发作后长达24小时,在所研究的任何区域中,突触素II的mRNA水平均无显著变化,并且推测突触素II与点燃几乎无关。我们认为,与点燃相关的双侧齿状回中突触素I的mRNA水平局部升高表明,在齿状颗粒细胞参与的突触回路中发生了兴奋性变化。突触素I可能参与了点燃中神经元可塑性的突触前分子机制。

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验