Velho Tarciso A F, Mello Claudio V
Department of Behavioral Neuroscience, Oregon Health and Science University, Beaverton, Oregon 97006, USA.
J Neurosci. 2008 Nov 12;28(46):11871-82. doi: 10.1523/JNEUROSCI.2307-08.2008.
The consolidation of long-lasting sensory memories requires the activation of gene expression programs in the brain. Despite considerable knowledge about the early components of this response, little is known about late components (i.e., genes regulated 2-6 h after stimulation) and the relationship between early and late genes. Birdsong represents one of the best natural behaviors to study sensory-induced gene expression in awake, freely behaving animals. Here we show that the expression of several isoforms of synapsins, a group of phosphoproteins thought to regulate the dynamics of synaptic vesicle storage and release, is induced by auditory stimulation with birdsong in the caudomedial nidopallium (NCM) of the zebra finch (Taeniopygia guttata) brain. This induction occurs mainly in excitatory (non-GABAergic) neurons and is modulated (suppressed) by early song-inducible proteins. We also show that ZENK, an early song-inducible transcription factor, interacts with the syn3 promoter in vivo, consistent with a direct regulatory effect and an emerging novel view of ZENK action. These results demonstrate that synapsins are a late component of the genomic response to neuronal activation and that their expression depends on a complex set of regulatory interactions between early and late regulated genes.
持久感觉记忆的巩固需要激活大脑中的基因表达程序。尽管对这一反应的早期组成部分已有相当多的了解，但对晚期组成部分（即刺激后2 - 6小时被调控的基因）以及早期和晚期基因之间的关系却知之甚少。鸟鸣是研究清醒、自由活动动物中感觉诱导基因表达的最佳自然行为之一。在这里，我们表明，突触素的几种亚型的表达，这是一组被认为调节突触小泡储存和释放动态的磷蛋白，在斑胸草雀（Taeniopygia guttata）大脑的内侧巢核（NCM）中由鸟鸣的听觉刺激诱导产生。这种诱导主要发生在兴奋性（非GABA能）神经元中，并受到早期歌曲诱导蛋白的调节（抑制）。我们还表明，早期歌曲诱导转录因子ZENK在体内与syn3启动子相互作用，这与直接调控作用以及ZENK作用的一种新出现的观点一致。这些结果表明，突触素是神经元激活的基因组反应的晚期组成部分，并且它们的表达取决于早期和晚期调控基因之间复杂的调控相互作用。
