Kornmann M, Ishiwata T, Arber N, Beger H G, Korc M
Department of Medicine, University of California, Irvine 92697, USA.
Pancreas. 1998 Aug;17(2):158-62. doi: 10.1097/00006676-199808000-00008.
Cyclin D1 is an important regulator of the G1 phase of the cell cycle. In this study we examined the expression and localization of cyclin D1 in chronic pancreatitis (CP), a condition associated with fibrosis, acinar cell degeneration, and foci of duct cell proliferation. Northern blotting of total RNA from the normal pancreas and from the pancreas of patients with CP revealed the presence of a 4.4-kb cyclin D1 mRNA transcript in all samples. Densitometric analysis indicated that pancreatic cyclin D1 mRNA levels were 2.0-fold increased in CP compared to normal controls (p = 0.0046). In the normal pancreas, faint cyclin D1 immunoreactivity was present in the nuclei and cytoplasm of some ductal cells and islet cells. In contrast, in CP, moderate to strong cyclin D1 immunoreactivity was present in many ductal and islet cells, as well as in some acinar cells and fibroblasts. These findings raise the possibility that enhanced cyclin D1 expression may contribute to a hyperproliferative state in CP.
细胞周期蛋白D1是细胞周期G1期的重要调节因子。在本研究中，我们检测了细胞周期蛋白D1在慢性胰腺炎（CP）中的表达和定位，慢性胰腺炎与纤维化、腺泡细胞变性及导管细胞增殖灶有关。对正常胰腺和慢性胰腺炎患者胰腺的总RNA进行Northern印迹分析，结果显示所有样本中均存在4.4 kb的细胞周期蛋白D1 mRNA转录本。光密度分析表明，与正常对照组相比，慢性胰腺炎患者胰腺中细胞周期蛋白D1 mRNA水平增加了2.0倍（p = 0.0046）。在正常胰腺中，一些导管细胞和胰岛细胞的细胞核及细胞质中存在微弱的细胞周期蛋白D1免疫反应性。相比之下，在慢性胰腺炎中，许多导管细胞和胰岛细胞以及一些腺泡细胞和成纤维细胞中存在中度至强的细胞周期蛋白D1免疫反应性。这些发现提示细胞周期蛋白D1表达增强可能导致慢性胰腺炎的增殖亢进状态。
