来自富含根毛的蒺藜苜蓿cDNA文库的表达序列标签。
Expressed sequence tags from a root-hair-enriched medicago truncatula cDNA library.
作者信息
Covitz PA, Smith LS, Long SR
机构信息
Department of Biological Sciences (P.A.C., L.S.S., S.R.L.).
出版信息
Plant Physiol. 1998 Aug;117(4):1325-32. doi: 10.1104/pp.117.4.1325.
Abstract
The root hair is a specialized cell type involved in water and nutrient uptake in plants. In legumes the root hair is also the primary site of recognition and infection by symbiotic nitrogen-fixing Rhizobium bacteria. We have studied the root hairs of Medicago truncatula, which is emerging as an increasingly important model legume for studies of symbiotic nodulation. However, only 27 genes from M. truncatula were represented in GenBank/EMBL as of October, 1997. We report here the construction of a root-hair-enriched cDNA library and single-pass sequencing of randomly selected clones. Expressed sequence tags (899 total, 603 of which have homology to known genes) were generated and made available on the Internet. We believe that the database and the associated DNA materials will provide a useful resource to the community of scientists studying the biology of roots, root tips, root hairs, and nodulation.
摘要
根毛是植物中参与水分和养分吸收的一种特化细胞类型。在豆科植物中，根毛也是共生固氮根瘤菌识别和侵染的主要部位。我们研究了蒺藜苜蓿的根毛，它正逐渐成为共生结瘤研究中越来越重要的豆科模式植物。然而，截至1997年10月，GenBank/EMBL中仅收录了27个蒺藜苜蓿基因。我们在此报告了一个根毛富集cDNA文库的构建以及对随机选择克隆的单通道测序。生成了表达序列标签（共899个，其中603个与已知基因具有同源性）并在互联网上提供。我们相信该数据库及相关DNA材料将为研究根、根尖、根毛和结瘤生物学的科学家群体提供有用资源。
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