叶绿体中异源rRNA操纵子启动子的转录揭示了对特定激活因子的需求。
Transcription from heterologous rRNA operon promoters in chloroplasts reveals requirement for specific activating factors.
作者信息
Sriraman P, Silhavy D, Maliga P
机构信息
Waksman Institute, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, New Jersey 08854-8020, USA.
出版信息
Plant Physiol. 1998 Aug;117(4):1495-9. doi: 10.1104/pp.117.4.1495.
Abstract
The plastid rRNA (rrn) operon in chloroplasts of tobacco (Nicotiana tabacum), maize, and pea is transcribed by the plastid-encoded plastid RNA polymerase from a sigma70-type promoter (P1). In contrast, the rrn operon in spinach (Spinacia oleracea) and mustard chloroplasts is transcribed from the distinct Pc promoter, probably also by the plastid-encoded plastid RNA polymerase. Primer-extension analysis reported here indicates that in Arabidopsis both promoters may be active. To understand promoter selection in the plastid rrn operon in the different species, we have tested transcription from the spinach rrn promoter in transplastomic tobacco and from the tobacco rrn promoter in transplastomic Arabidopsis. Our data suggest that transcription of the rrn operon depends on species-specific factors that facilitate transcription initiation by the general transcription machinery.
摘要
烟草（Nicotiana tabacum）、玉米和豌豆叶绿体中的质体rRNA（rrn）操纵子由质体编码的质体RNA聚合酶从sigma70型启动子（P1）转录。相比之下，菠菜（Spinacia oleracea）和芥菜叶绿体中的rrn操纵子从不同的Pc启动子转录，可能也是由质体编码的质体RNA聚合酶转录。本文报道的引物延伸分析表明，在拟南芥中两个启动子可能都有活性。为了解不同物种质体rrn操纵子中的启动子选择，我们测试了转质体烟草中转录自菠菜rrn启动子的转录情况以及转质体拟南芥中转录自烟草rrn启动子的转录情况。我们的数据表明，rrn操纵子的转录取决于物种特异性因子，这些因子可促进通用转录机制的转录起始。
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