Tohdoh N, Shinozaki K, Sugiura M
Nucleic Acids Res. 1981 Oct 24;9(20):5399-406. doi: 10.1093/nar/9.20.5399.
The nucleotide sequence of the segment of tobacco chloroplast DNA adjacent to and including the start of the 16S rRNA gene has been determined. The region just preceding this gene was found to contain a tRNAVal gene and promoter-type sequences similar to those which occur in E. coli were found before this tRNA gene. E. coli RNA polymerase can recognize these sequences and in vitro co-transcribes the tRNA and rRNA genes.
已确定烟草叶绿体DNA中与16S rRNA基因起始位点相邻并包含该起始位点的片段的核苷酸序列。发现该基因之前的区域含有一个tRNAVal基因,并且在该tRNA基因之前发现了与大肠杆菌中出现的序列类似的启动子型序列。大肠杆菌RNA聚合酶能够识别这些序列,并在体外共同转录tRNA和rRNA基因。