Amaechi B T, Higham S M, Edgar W M
Cariology Research Group, Department of Clinical Dental Sciences, University of Liverpool, UK.
Caries Res. 1998;32(5):351-6. doi: 10.1159/000016471.
The aim of the present study was to develop transverse microradiography (TMR) in order to quantitatively assess the influence of artificial saliva and orange juice on the erosion of bovine enamel. Bovine incisors were sectioned sagittally into two equal halves. Each half was coated with acid-resistant nail varnish except for a rectangular enamel window on the labial surface of the tooth. While both halves of each tooth were immersed in pure orange juice 6 times daily for a period of 5 min at each occasion making a total of 30 min per day, one half was stored in artificial saliva and the other half in deionized distilled water between exposures to orange juice and for the remaining 12 h overnight, for 24 days making a total of 12 h of exposure to orange juice. Sections bearing intact and eroded enamel surfaces were cut from each specimen and ground to 80 microm thickness, and TMR of the sections was made. Mineral loss was quantified from the microradiographs using two-step image analysis. Mineral loss was significantly greater in those specimens cycled in orange juice and de-ionized distilled water. TMR was used successfully to quantify the mineral loss by erosion in vitro, and it is envisaged that it will be useful for specimens used in intra-oral appliances during in situ trials. Artificial saliva saturated with respect to calcium and phosphate salts can possibly remineralize an erosive lesion and may reduce the degree of erosion.
本研究的目的是开发横向显微放射照相术（TMR），以便定量评估人工唾液和橙汁对牛牙釉质侵蚀的影响。将牛切牙矢状切成两个相等的部分。每一部分除牙齿唇面的一个矩形釉质窗口外，均涂有耐酸指甲油。每颗牙齿的两部分每天6次浸入纯橙汁中，每次浸泡5分钟，每天共计30分钟，在两次接触橙汁之间以及其余12小时的夜间，其中一半保存在人工唾液中，另一半保存在去离子蒸馏水中，持续24天，总共接触橙汁12小时。从每个标本上切下带有完整和侵蚀釉质表面的切片，并研磨至80微米厚，然后对切片进行TMR检查。使用两步图像分析从显微放射照片中量化矿物质损失。在橙汁和去离子蒸馏水中循环处理的标本中，矿物质损失明显更大。TMR已成功用于体外定量侵蚀造成的矿物质损失，并且预计它将对原位试验中口腔内装置使用的标本有用。相对于钙盐和磷酸盐饱和的人工唾液可能会使侵蚀性病变再矿化，并可能降低侵蚀程度。
