Amaechi B T, Higham S M, Edgar W M
Department of Clinical Dental Sciences, School of Dentistry, University of Liverpool, U.K.
Arch Oral Biol. 1998 Feb;43(2):157-61. doi: 10.1016/s0003-9969(97)00109-x.
The aim was to determine the effect of xylitol, fluoride and xylitol/fluoride combined on the erosion of dental enamel by pure orange juice in vitro. Freshly extracted bovine incisors were sectioned vertically into four equal portions. Each portion was then coated with an acid-resistant nail varnish except for an enamel window on the labial surface of the tooth. These were then divided into four groups with each group containing one portion of each tooth selected randomly. Four erosive agents were prepared as follows: (A) pure orange juice only; pure orange juice plus either (B) xylitol (25% w/v) or (C) fluoride (0.5 parts/10(6)) or D) xylitol/fluoride (25% and 0.5 parts/10(6) respectively). Each group was assigned to one of the erosive agents and immersed six times daily for a period of 5 min on each occasion and stored in artificial saliva between exposures and for 12 hr overnight, for 24 days making a total of 12 hr of exposure to the assigned erosive agent. Sections were cut from each enamel specimen ground to a thickness of 80 microns and microradiographed. Mineral loss was quantified by a two-step image analysis. Mineral loss was significantly lower (p < 0.05) in group D (xylitol/fluoride) only when compared with group A (pure orange juice only). The numerical values of mineral loss could be ranked as follows: group D < group C < group B < group A. It was concluded that xylitol and fluoride have an additive effect in the reduction of dental erosion by pure orange juice in vitro.
目的是确定木糖醇、氟化物以及木糖醇/氟化物组合对纯橙汁在体外侵蚀牙釉质的影响。将新鲜拔除的牛切牙垂直切成四个相等部分。然后，除牙齿唇面的一个牙釉质窗口外，每个部分都涂上耐酸指甲油。然后将这些部分随机分为四组，每组包含随机选择的每颗牙齿的一个部分。制备四种侵蚀剂如下：（A）仅纯橙汁；纯橙汁加（B）木糖醇（25% w/v）或（C）氟化物（0.5 份/10⁶）或（D）木糖醇/氟化物（分别为 25%和 0.5 份/10⁶）。每组被分配一种侵蚀剂，每天浸泡六次，每次浸泡 5 分钟，并在每次浸泡之间以及过夜 12 小时期间储存在人工唾液中，持续 24 天，总共暴露于指定侵蚀剂 12 小时。从每个牙釉质标本上切下厚度为 80 微米的切片并进行显微放射照相。通过两步图像分析对矿物质损失进行量化。仅与 A 组（仅纯橙汁）相比时，D 组（木糖醇/氟化物）的矿物质损失显著更低（p < 0.05）。矿物质损失的数值排序如下：D 组 < C 组 < B 组 < A 组。得出的结论是，木糖醇和氟化物在体外对纯橙汁减少牙釉质侵蚀方面具有相加作用。
