Burdelya L G, Grosheva I A, Dyakova N A, Deichman G I
Institute of Carcinogenesis, Cancer Research Center, Russian Academy of Medical Science, Moscow, Russia.
Tumour Biol. 1998;19(5):346-55. doi: 10.1159/000030027.
The ability of nonactivated peritoneal macrophages to induce nitric oxide (NO) secretion in transformed and tumor cells of the same origin differing in tumorigenic (TGA) and spontaneous metastatic activities (SMA) was examined. Low tumorigenic and nonmetastatic spontaneously transformed in vitro hamster embryo cells of the STHE strain in contact with macrophages, or their non-purified soluble product were the highest producers of NO. In vivo selected STHE cell variants characterized by middle or high TGA demonstrated low, or no NO production, respectively (independently of SMA- or SMA+). Two highly tumorigenic RSV-SR (v-src) transformed cell strains (SMA- and SMA+) were both negative in NO production. Thus, NO production by tumor cells appeared to be inversely correlated with TGA level and less dependent on SMA.
研究了未活化的腹膜巨噬细胞在致瘤性（TGA）和自发转移活性（SMA）不同的同源转化细胞和肿瘤细胞中诱导一氧化氮（NO）分泌的能力。与巨噬细胞或其未纯化的可溶性产物接触的STHE品系低致瘤性和非转移性的体外自发转化仓鼠胚胎细胞是NO的最高产生者。体内选择的具有中等或高TGA特征的STHE细胞变体分别显示出低NO产生或不产生NO（与SMA-或SMA+无关）。两种高致瘤性的RSV-SR（v-src）转化细胞株（SMA-和SMA+）在NO产生方面均为阴性。因此，肿瘤细胞产生NO似乎与TGA水平呈负相关，且对SMA的依赖性较小。
