Rye P D, Bovin N V, Vlasova E V, Molodyk A A, Baryshnikov A, Kreutz F T, Garinther W I, Schultes B C, Noujaim A A, Madiyalakan R, Magnani J, Nilsson O, Nilsson K, Nustad K, Norum L, Bell H, Cao Y, Suresh M R, Very D L, Freeman J V, Yeung K K, Hilgers J
Central Laboratory, Norwegian Radium Hospital, Montebello, Oslo, Norway.
Tumour Biol. 1998;19(5):390-420. doi: 10.1159/000030032.
The ISOBM TD-6 Workshop is the first international workshop on monoclonal antibodies against the Sialyl Lewisa (SLea) antigen. Eight research groups participated in a blind study to characterize the epitope binding, relative affinity and performance in immunoradiometric assays, of a panel of 20 monoclonal antibodies. The antibodies were tested against a diverse panel of neoglycoconjugates, purified antigens and human serum pools from gastrointestinal malignancies. Epitope specificities were determined for the majority of antibodies in the panel. Cross-reactivity with related saccharide structures was noted in several antibodies. Overall, the results of the TD-6 Workshop show further development of SLea immunoassays may yield yet more specific assays for the detection and management of gastrointestinal and other malignancies.
国际单克隆抗体标准物（ISOBM）TD-6研讨会是首个关于抗唾液酸化路易斯抗原a（SLea）单克隆抗体的国际研讨会。八个研究小组参与了一项盲法研究，以表征一组20种单克隆抗体的表位结合、相对亲和力以及免疫放射分析中的性能。这些抗体针对多种新糖缀合物、纯化抗原以及来自胃肠道恶性肿瘤患者的混合人血清进行了检测。确定了该组中大多数抗体的表位特异性。在几种抗体中发现了与相关糖类结构的交叉反应性。总体而言，TD-6研讨会的结果表明，SLea免疫分析的进一步发展可能会产生更具特异性的检测方法，用于胃肠道及其他恶性肿瘤的检测和管理。
