Li J, Takeda H, Tsuji M, Liu L, Matsumiya T
Department of Pharmacology, Tokyo Medical College, Japan.
Methods Find Exp Clin Pharmacol. 1998 Jun;20(5):409-17. doi: 10.1358/mf.1998.20.5.485702.
The present study was conducted to test the hypothesis that central corticotropin-releasing factor (CRF) systems mediate the changes in the dynamics of brain noradrenergic and serotonergic systems induced by electric footshock stress. Serum corticosterone concentrations were determined fluorometrically and brain monoamine contents (noradrenaline, serotonin and their metabolites) in five brain regions in rats were measured by high-performance liquid chromatography with electrochemical detection 10 and 40 min after exposure to electric footshock stress for 1 hour. Intracerebroventricular administration of a CRF antagonist, alpha-helical CRF (100 micrograms), had no effect on serum corticosterone concentrations in either normal or stressed animals. However, electric footshock stress-induced increases in noradrenaline turnover were significantly reduced by the administration of alpha-helical CRF9-41, in the amygdala and septum at both time points after exposure to electric footshock stress. In contrast, electric footshock stress-induced increases in serotonin turnover in all of the brain regions were further elevated by the administration of alpha-helical CRF 9-41, in almost all of the brain regions examined. These results suggest that central CRF neuronal systems are involved in mediating the electric footshock stress-induced changes in the dynamics of brain noradrenergic and serotonergic systems in rats.
中枢促肾上腺皮质激素释放因子（CRF）系统介导电足部电击应激诱导的脑去甲肾上腺素能和5-羟色胺能系统动力学变化。通过荧光法测定血清皮质酮浓度，并在大鼠暴露于1小时电足部电击应激后10分钟和40分钟，采用高效液相色谱-电化学检测法测量大鼠五个脑区的脑单胺含量（去甲肾上腺素、5-羟色胺及其代谢产物）。脑室内注射CRF拮抗剂α-螺旋CRF（100微克）对正常或应激动物的血清皮质酮浓度均无影响。然而，在暴露于电足部电击应激后的两个时间点，杏仁核和隔区注射α-螺旋CRF9-41可显著降低电足部电击应激诱导的去甲肾上腺素周转率增加。相反，在几乎所有检测的脑区，注射α-螺旋CRF 9-41可使电足部电击应激诱导的所有脑区5-羟色胺周转率进一步升高。这些结果表明，中枢CRF神经元系统参与介导大鼠电足部电击应激诱导的脑去甲肾上腺素能和5-羟色胺能系统动力学变化。
