Fonina L A, Gur'ianov S A, Efremov M A, Smirnova O V
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Bioorg Khim. 1998 Jun;24(6):403-7.
A new method was developed for the isolation of biologically active peptides present in small quantities in supernatants of bone marrow cell cultures. Four new peptides, which exhibited immunostimulating and differentiating activities, were isolated from the supernatant using solid phase extraction and reversed-phase HPLC. For these peptides, the following primary structures were elucidated: Leu-Val-Cys-Tyr-Pro-Gln, Phe-Arg-Pro-Arg-Ile-Met-Thr-Pro, Val-Val-Tyr-Pro-Asp, and Val-Asp-Pro-Pro.
开发了一种新方法，用于从骨髓细胞培养上清液中分离少量存在的生物活性肽。使用固相萃取和反相高效液相色谱法从该上清液中分离出四种具有免疫刺激和分化活性的新肽。对于这些肽，阐明了以下一级结构：亮氨酸-缬氨酸-半胱氨酸-酪氨酸-脯氨酸-谷氨酰胺、苯丙氨酸-精氨酸-脯氨酸-精氨酸-异亮氨酸-甲硫氨酸-苏氨酸-脯氨酸、缬氨酸-缬氨酸-酪氨酸-脯氨酸-天冬氨酸以及缬氨酸-天冬氨酸-脯氨酸-脯氨酸。
