四氧嘧啶-钒组合对脂质过氧化和抗氧化酶活性的体外作用。

In vitro effects of alloxan-vanadium combination on lipid peroxidation and on antioxidant enzyme activity.

Alexandrova A, Kirkova M, Russanov E

Institute of Physiology, Bulgarian Academy of Sciences, Sofia.

Gen Pharmacol. 1998 Sep;31(3):489-93. doi: 10.1016/s0306-3623(97)00452-7.

Abstract

The in vitro effects of alloxan, dialuric acid and vanadium ions, alone or in combination, on lipid peroxidation and on antioxidant enzyme activity in rat liver and kidney were studied. 2. Unlike alloxan, alloxan-glutathione (GSH) and dialuric acid increased lipid peroxidation, which could be explained by the decreased activity of catalase and GSH peroxidase during incubation. 3. Vanadium(IV) ions increased the amount of thiobarbituric acid-reacting substances, but neither vanadium(IV) nor vanadium(V) changed the enzyme activity. 4. The combination of vanadium ions and alloxan-GSH or dialuric acid had no additive effect on lipid peroxidation. Vanadium ions decreased the dialuric acid-induced inhibition of catalase activity. 5. The present results suggest the therapeutic value of vanadium as an antidiabetic agent.

摘要

研究了四氧嘧啶、双脲酸和钒离子单独或联合作用对大鼠肝脏和肾脏脂质过氧化及抗氧化酶活性的体外影响。2. 与四氧嘧啶不同，四氧嘧啶 - 谷胱甘肽（GSH）和双脲酸会增加脂质过氧化，这可以通过孵育过程中过氧化氢酶和GSH过氧化物酶活性的降低来解释。3. 钒（IV）离子增加了硫代巴比妥酸反应物质的量，但钒（IV）和钒（V）均未改变酶活性。4. 钒离子与四氧嘧啶 - GSH或双脲酸的组合对脂质过氧化没有相加作用。钒离子降低了双脲酸诱导的过氧化氢酶活性抑制。5. 目前的结果表明钒作为抗糖尿病药物的治疗价值。