Niezen J H, Waghorn G C, Charleston W A
AgResearch, Grasslands, Palmerston North, New Zealand.
Vet Parasitol. 1998 Jul 17;78(1):13-21. doi: 10.1016/s0304-4017(98)00121-6.
Forty four 12-14 week old Polled Dorset ram lambs, raised helminth free from birth, were used to investigate the effects of condensed tannins (CT) in lotus (Lotus pedunculatus) on lamb growth and gastrointestinal nematode establishment and fecundity. Condensed tannins bind to proteins in the rumen and increase the flow of protein to the intestines. Lambs were allocated to either a ryegrass (Lolium perenne) or lotus diet fed ad libitum, with or without twice daily drenching of polyethylene glycol (PEG) which binds with and deactivates the CT. One week after allocation to the diets, each lamb was infected per os with 10,000 Ostertagia circumcincta and 10,000 Trichostrongylus colubriformis larvae and slaughtered 28 or 29 days later. Lambs fed lotus had lower faecal egg counts (P < 0.0001) and lower O. circumcincta burdens (P < 0.001), fewer female O. circumcincta (P < 0.0001) and higher faecal dry matter (P < 0.001) than lambs fed ryegrass, but numbers of T. colubriformis nematodes were not affected. This trial did not ascribe beneficial effects of L. pedunculatus to CT per se and the use of PEG does not seem appropriate to studies of parasitology.
44只12 - 14周龄的无角陶赛特公羔羊，自出生起就饲养在无蠕虫环境中，用于研究百脉根（Lotus pedunculatus）中的缩合单宁（CT）对羔羊生长、胃肠道线虫定殖和繁殖力的影响。缩合单宁与瘤胃中的蛋白质结合，增加蛋白质向肠道的流动。羔羊被随意分配到黑麦草（Lolium perenne）或百脉根日粮组，每组又分为添加或不添加每日两次灌服聚乙二醇（PEG）的组，PEG可与CT结合并使其失活。在分配到日粮一周后，每只羔羊经口感染10,000条环形奥斯特线虫（Ostertagia circumcincta）和10,000条蛇形毛圆线虫（Trichostrongylus colubriformis）幼虫，并在28或29天后屠宰。与饲喂黑麦草的羔羊相比，饲喂百脉根的羔羊粪便虫卵计数更低（P < 0.0001）、环形奥斯特线虫负荷更低（P < 0.001）、雌性环形奥斯特线虫数量更少（P < 0.0001）且粪便干物质含量更高（P < 0.001），但蛇形毛圆线虫数量未受影响。该试验并未将百脉根的有益作用归因于CT本身，并且PEG的使用在寄生虫学研究中似乎并不合适。
