Sapino A, Cassoni P, Stella A, Bussolati G
Department of Biomedical Sciences and Oncology, University of Torino, Italy.
Anticancer Res. 1998 May-Jun;18(3C):2181-6.
In the present work, first we reviewed and completed our previous experiments on the antiproliferative effect of oxytocin (OT) in breast cancer cell lines. In vitro, OT 10 nM and 100 nM inhibited cell proliferation of MDA-MB231 (human breast carcinoma) and TS/A (mouse mammary carcinoma) cell lines. In vivo, OT significantly reduced the growth of TS/A mammary tumors. Both effects are mediated by specific receptors (OTR) distributed on cell surface. Second, using immunohistochemistry and RT-PCR we detected OTR and OTR mRNA in normal and pathological breast tissue. There is no correlation among OTR presence in breast carcinomas and the age of patients, tumor stage, estrogen receptor positivity, oncogene expression and proliferation rate of the same tumor. On the contrary, progesterone and OTR expression are correlated. These data confirm our previous evidence of a role of OT and OTR in normal and neoplastic breast cells.
在本研究中，首先我们回顾并完成了我们之前关于催产素（OT）对乳腺癌细胞系抗增殖作用的实验。在体外，10 nM和100 nM的OT抑制了MDA-MB231（人乳腺癌）和TS/A（小鼠乳腺癌）细胞系的细胞增殖。在体内，OT显著降低了TS/A乳腺肿瘤的生长。这两种作用均由分布在细胞表面的特异性受体（OTR）介导。其次，我们使用免疫组织化学和逆转录聚合酶链反应（RT-PCR）检测了正常和病理乳腺组织中的OTR和OTR mRNA。乳腺癌中OTR的存在与患者年龄、肿瘤分期、雌激素受体阳性、癌基因表达以及同一肿瘤的增殖率之间没有相关性。相反，孕激素和OTR表达是相关的。这些数据证实了我们之前关于OT和OTR在正常和肿瘤性乳腺细胞中作用的证据。
