Bussolati G, Cassoni P, Ghisolfi G, Negro F, Sapino A
Department of Biomedical Sciences and Oncology, University of Torino, Torino, Italy.
Am J Pathol. 1996 Jun;148(6):1895-903.
Recent evidence indicates that oxytocin (OT), in addition to the induction of myometrial and myoepithelial cell contraction, can influence proliferation and differentiation in developing mammary glands and in breast cancer cells, hence the interest in detecting and locating OT receptors (OTRs). We produced rabbit antisera and a monoclonal antibody against a synthetic peptide corresponding to the carboxy terminus of the predicted OTR sequence. We tested their specificity in immunoblasts and immunocytochemical tests. All of the antibodies specifically stained myometrium (at term of pregnancy). In the human breast, OTRs were detected in myoepithelial cells along ducts of normal lobules and in sclerosing adenosis. Intraductal cells in benign hyperplastic lesions were also positive. OTRs were demonstrated in cases of primary and metastatic carcinomas of the breast. In the same tissues, OTR gene expression was shown by reverse transcriptase polymerase chain reaction procedures detecting the specific mRNA. These results suggest that the interaction between OT and its receptors might play a role in the origin and evolution of non-neoplastic lesions and carcinomas of the breast.
最近的证据表明，催产素（OT）除了能诱导子宫肌层和肌上皮细胞收缩外，还能影响发育中的乳腺和乳腺癌细胞的增殖与分化，因此人们对检测和定位OT受体（OTRs）产生了兴趣。我们制备了针对与预测的OTR序列羧基末端相对应的合成肽的兔抗血清和单克隆抗体。我们在免疫母细胞和免疫细胞化学试验中测试了它们的特异性。所有抗体均特异性地染色子宫肌层（妊娠末期）。在人类乳腺中，在正常小叶导管周围的肌上皮细胞以及硬化性腺病中检测到了OTRs。良性增生性病变中的导管内细胞也呈阳性。在原发性和转移性乳腺癌病例中也证实了OTRs的存在。在相同组织中，通过逆转录聚合酶链反应程序检测特异性mRNA，显示了OTR基因的表达。这些结果表明，OT与其受体之间的相互作用可能在乳腺非肿瘤性病变和癌的发生及发展中起作用。
