Kojima S, Matsuki O, Nomura T, Kubodera A, Yamaoka K
Research Institute for Biological Sciences, Science University of Tokyo, Chiba, Japan.
Anticancer Res. 1998 Jul-Aug;18(4A):2471-6.
We examined the elevation of glutathione (GSH) levels in the liver of C57BL/6 female mice after low-dose r-ray irradiation and its inhibitory effect on CClI4-induced liver damage. The liver GSH level increased soon after irradiation with 50 cGy of gamma-rays, reached a maximum at around 12 post-treatment, and returned almost to the control level by 24 h. The activities of glutathione reductase, and glutathione peroxidase also showed the same pattern of change, while the activity of gamma-glutamylcysteine synthetase showed a gradual increase up to 24 h. The effect of pre-irradiation on CCl4-induced liver damage was also investigated. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in serum were markedly increased 12 h post-treatment with CCl4. Both increases were significantly suppressed by a single low-dose pre-irradiation. Malondialdehyde, a marker of lipidperoxidation, was also greatly elevated after CCl4 treatment, and its increase was suppressed by irradiation. These results suggest low-dose gamma-ray irradiation might be effective for the prevention of and/or therapy of various reactive oxygen species-related diseases including cancer.
我们研究了低剂量γ射线照射后C57BL/6雌性小鼠肝脏中谷胱甘肽（GSH）水平的升高及其对四氯化碳诱导的肝损伤的抑制作用。用50 cGy的γ射线照射后，肝脏GSH水平很快升高，在处理后约12小时达到最大值，并在24小时时几乎恢复到对照水平。谷胱甘肽还原酶和谷胱甘肽过氧化物酶的活性也呈现相同的变化模式，而γ-谷氨酰半胱氨酸合成酶的活性在24小时内逐渐增加。还研究了预照射对四氯化碳诱导的肝损伤的影响。用四氯化碳处理后12小时，血清中谷氨酸草酰乙酸转氨酶和谷氨酸丙酮酸转氨酶的活性显著增加。单次低剂量预照射均能显著抑制这两种酶活性的增加。脂质过氧化的标志物丙二醛在四氯化碳处理后也大幅升高，其升高受到照射的抑制。这些结果表明，低剂量γ射线照射可能对预防和/或治疗包括癌症在内的各种活性氧相关疾病有效。
