Satoh K, Anzai S, Sakagami H
Analysis Center, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Anticancer Res. 1998 Jul-Aug;18(4A):2749-53.
The radical modulation activity of hot water and NaOH extracts of the thorn apple of Crataegus Cuneata Sieb. et. Zucc. was investigated, using ESR spectroscopy. The radical intensity of sodium ascorbate was slightly reduced at lower concentrations of the extracts, but it was synergistically enhanced at higher concentrations. Higher concentrations of the extracts also synergistically stimulated the cytotoxic action of sodium ascorbate against human oral squamous carcinoma and salivary gland tumor cell lines. All these extracts effectively scavenged superoxide anion and hydroxyl radical, produced by hypoxanthine-xanthine oxidase and Fenton reaction, respectively, and their activity was comparable with that of lignins prepared by the same procedure from other plants. These data further supports the medicinal efficacy of plant extracts.
利用电子自旋共振光谱法研究了山楂叶悬钩子热水提取物和氢氧化钠提取物的自由基调节活性。在较低浓度提取物作用下，抗坏血酸钠的自由基强度略有降低，但在较高浓度时会协同增强。较高浓度的提取物还能协同刺激抗坏血酸钠对人口腔鳞状细胞癌和唾液腺肿瘤细胞系的细胞毒性作用。所有这些提取物都能有效清除分别由次黄嘌呤-黄嘌呤氧化酶和芬顿反应产生的超氧阴离子和羟基自由基，其活性与用相同方法从其他植物中制备的木质素相当。这些数据进一步支持了植物提取物的药用功效。
