Meghdadi S, Karanikas G, Schlagbauer-Wadl H, Jansen B, Chehne F, Rodrigues M, Pehamberger H, Sinzinger H
Department of Nuclear Medicine, University of Vienna, Austria.
Anticancer Res. 1998 Jul-Aug;18(4A):2759-62.
The aim of our study was to examine whether technetium-99m 1,2-bis[bis(2-ethoxyethyl) phosphino]ethane (tetrofosmin) a lipophilic, cationic tracer which was first developed for myocardial perfusion imaging, could be a new radiopharmaceutical for melanoma imaging. We therefore used two human cell lines, SK-MEL 28 and 5i8 A2 (n = 6, cell concentration 106/ml, incubation at 22 degrees C and 37 degrees C, 50-100 approximately lCi/ml Tc-99m-tetrofosmin, time of incubation 10-180 minutes). The cellular uptake by both cell lines was determined. In contrast to another non- melanoma tumor cell line MCF-7 (a human adenocarcinoma breast cancer) which reached steadystate almost immediately (within 10 minutes), the cellular uptake of SK-MEL-28 increased after 60 minutes and showed a very high uptake (> 10%) after 120 minutes and decreased after 180 minutes (6-8%), while the uptake in 518 A2 cells was about 5% after 90 minutes. Our data show that Tc-99m-tetrofosmin could be a promising agent for melanoma imaging.
我们研究的目的是检验锝-99m 1,2-双[双(2-乙氧基乙基)膦基]乙烷（替曲膦），一种最初开发用于心肌灌注显像的亲脂性阳离子示踪剂，是否可能成为用于黑色素瘤显像的新型放射性药物。因此，我们使用了两种人类细胞系，SK-MEL 28和5i8 A2（n = 6，细胞浓度为106/ml，在22℃和37℃孵育，50 - 100约lCi/ml的锝-99m-替曲膦，孵育时间为10 - 180分钟）。测定了两种细胞系的细胞摄取情况。与另一种非黑色素瘤肿瘤细胞系MCF-7（人腺癌乳腺癌）几乎立即（在10分钟内）达到稳态不同，SK-MEL-28的细胞摄取在60分钟后增加，在120分钟后显示出非常高的摄取（>10%），并在180分钟后下降（6 - 8%），而518 A2细胞在90分钟后的摄取约为5%。我们的数据表明，锝-99m-替曲膦可能是一种有前景的黑色素瘤显像剂。
