Felici F, Luzzago A, Monaci P, Nicosia A, Sollazzo M, Traboni C
IRBM (Istituto di Biologia Molecolare P. Angeletti), Rome, Italy.
Biotechnol Annu Rev. 1995;1:149-83. doi: 10.1016/s1387-2656(08)70051-6.
The isolation of ligands that bind biologically relevant molecules is fundamental to the understanding of biological processes and to the search for therapeutics. Filamentous phage can be used to display foreign peptides and proteins in physical association with their DNA coding sequences. Repertoires larger than 10(8) phage clones expressing different peptide sequences can be prepared using molecular genetic techniques. The strategies utilizing this technology promise to provide not only new binding and possibly catalytic activities, but also lead structures for the development of new drugs and vaccines.
分离与生物相关分子结合的配体对于理解生物过程和寻找治疗方法至关重要。丝状噬菌体可用于展示与其DNA编码序列物理关联的外源肽和蛋白质。使用分子遗传技术可以制备表达不同肽序列的超过10⁸个噬菌体克隆的文库。利用这项技术的策略有望不仅提供新的结合活性以及可能的催化活性，还能为新药和疫苗的开发提供先导结构。
