Malik P, Terry T D, Bellintani F, Perham R N
Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK.
FEBS Lett. 1998 Oct 2;436(2):263-6. doi: 10.1016/s0014-5793(98)01140-5.
Many small peptides can be displayed on every copy of the major coat protein in recombinant filamentous bacteriophages but larger peptides can only be accommodated in hybrid virions mixed with wild-type protein subunits. A peptide insert of 12 residues capable of display at high copy number in a hybrid virion was found to be incapable of supporting recombinant virion assembly, a defect that could not be overcome by over-expressing leader peptidase in the same Escherichia coli cell. In contrast, over-expressing leader peptidase did increase the copy number of two 9-residue peptides that were poorly incorporated into hybrid virions. The factors that limit peptide display are varied and not restricted to the early stages of viral assembly.
许多小肽可展示于重组丝状噬菌体的每个主要衣壳蛋白拷贝上，但较大的肽只能容纳于与野生型蛋白质亚基混合的杂交病毒粒子中。发现一个能够在杂交病毒粒子中以高拷贝数展示的12个残基的肽插入片段无法支持重组病毒粒子的组装，在同一大肠杆菌细胞中过表达前导肽酶也无法克服这一缺陷。相比之下，过表达前导肽酶确实增加了两个掺入杂交病毒粒子效率较低的9个残基肽的拷贝数。限制肽展示的因素多种多样，并不局限于病毒组装的早期阶段。
